Recombinant Anti-TORC2 antibody [EPR3384(2)] (ab109081)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3384(2)] to TORC2
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TORC2 antibody [EPR3384(2)]
See all TORC2 primary antibodies -
Description
Rabbit monoclonal [EPR3384(2)] to TORC2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293T, Raji, and A673 cell lysates; Human tonsil tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3384(2) -
Isotype
IgG -
Research areas
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Co-activators/co-repressors
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Co-activators/co-repressors
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109081 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (3) |
1/1000 - 1/10000. Predicted molecular weight: 73 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended. |
Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 73 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
Target
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Function
Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). -
Tissue specificity
Most abundantly expressed in the thymus. Present in both B and T lymphocytes. Highly expressed in HEK293T cells and in insulinomas. High levels also in spleen, ovary, muscle and lung, with highest levels in muscle. Lower levels found in brain, colon, heart, kidney, prostate, small intestine and stomach. Weak expression in liver and pancreas. -
Sequence similarities
Belongs to the TORC family. -
Post-translational
modificationsPhosphorylation/dephosphorylation states of Ser-171 are required for regulating transduction of CREB activity. TORCs are inactive when phosphorylated, and active when dephosphorylated at this site. This primary site of phosphorylation, is regulated by cAMP and calcium levels and is dependent on the phosphorylation of SIKs by LKB1. Both insulin and AMPK increase this phosphorylation, of TORC2 while glucagon suppresses it. -
Cellular localization
Cytoplasm. Nucleus. Translocated from the nucleus to the cytoplasm on interaction of the phosphorylated form with 14-3-3 protein. In response to cAMP levels and glucagon, relocated to the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 200186 Human
- Entrez Gene: 310615 Rat
- Omim: 608972 Human
- SwissProt: Q53ET0 Human
- SwissProt: Q3LRZ1 Rat
- Unigene: 406392 Human
- Unigene: 13599 Rat
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Alternative names
- CREB regulated transcription coactivator 2 antibody
- CREB-regulated transcription coactivator 2 antibody
- CRTC2 antibody
see all
Images
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Anti-TORC2 antibody [EPR3384(2)] (ab109081) at 1/5000 dilution + Ins-1, rat insulinoma cell line, nuclear cell lysate at 1200000 cells
Secondary
HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 73 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
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All lanes : Anti-TORC2 antibody [EPR3384(2)] (ab109081) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : Raji cell lysate
Lane 3 : A673 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 73 kDa -
ab109081 at 1/100 dilution staining Torc2 in Human tonsil by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab109081 has been referenced in 7 publications.
- Smith LIF et al. Activation and expression of endogenous CREB-regulated transcription coactivators (CRTC) 1, 2 and 3 in the rat adrenal gland. J Neuroendocrinol 33:e12920 (2021). PubMed: 33314405
- Fang X et al. LINC00998 functions as a novel tumor suppressor in acute myeloid leukemia via regulating the ZFP36 ring finger protein/mammalian target of rapamycin complex 2 axis. Bioengineered 12:10363-10372 (2021). PubMed: 34699314
- Li P et al. CRTC2 Is a Key Mediator of Amino Acid-Induced Milk Fat Synthesis in Mammary Epithelial Cells. J Agric Food Chem 67:10513-10520 (2019). PubMed: 31475823
- Shi S et al. CRTC2 promotes non-small cell lung cancer A549 migration and invasion in vitro. Thorac Cancer 9:136-141 (2018). PubMed: 29105369
- Akhmedov D et al. Gs-DREADD Knock-In Mice for Tissue-Specific, Temporal Stimulation of Cyclic AMP Signaling. Mol Cell Biol 37:N/A (2017). WB . PubMed: 28167604
- Tian X et al. CRTC2 enhances HBV transcription and replication by inducing PGC1a expression. Virol J 11:30 (2014). WB . PubMed: 24529027
- Fu J et al. The short isoform of the ubiquitin ligase NEDD4L is a CREB target gene in hepatocytes. PLoS One 8:e78522 (2013). WB ; Mouse . PubMed: 24147136