Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse macrophage cell line)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
3 µg
Specification
Mouse macrophage cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA in TBST
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Goat Anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
20 second(s)
Bands
Specific: 72 kDa Non-specific: 70, 43, 48 kDa
Additional data
Abcam response
Antibody was tested as part of Abtrial as mouse is not listed on the datasheet as guaranteed application.
Abcam user community
Verified customer
Submitted Nov 12 2020