Anti-TORC2 (phospho S171) antibody (ab203187)
Key features and details
- Rabbit polyclonal to TORC2 (phospho S171)
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-TORC2 (phospho S171) antibody
See all TORC2 primary antibodies -
Description
Rabbit polyclonal to TORC2 (phospho S171) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide within Human TORC2 aa 150-250 (phospho S171) conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q53ET0 -
Positive control
- Human rectal carcinoma and gastric carcinoma lysates. Rat lung tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/100 - 1/1000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at 1/50 - 1/200 with fluorescent detection methods. |
Notes |
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WB
1/100 - 1/1000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa). |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use at 1/50 - 1/200 with fluorescent detection methods. |
Target
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Function
Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). -
Tissue specificity
Most abundantly expressed in the thymus. Present in both B and T lymphocytes. Highly expressed in HEK293T cells and in insulinomas. High levels also in spleen, ovary, muscle and lung, with highest levels in muscle. Lower levels found in brain, colon, heart, kidney, prostate, small intestine and stomach. Weak expression in liver and pancreas. -
Sequence similarities
Belongs to the TORC family. -
Post-translational
modificationsPhosphorylation/dephosphorylation states of Ser-171 are required for regulating transduction of CREB activity. TORCs are inactive when phosphorylated, and active when dephosphorylated at this site. This primary site of phosphorylation, is regulated by cAMP and calcium levels and is dependent on the phosphorylation of SIKs by LKB1. Both insulin and AMPK increase this phosphorylation, of TORC2 while glucagon suppresses it. -
Cellular localization
Cytoplasm. Nucleus. Translocated from the nucleus to the cytoplasm on interaction of the phosphorylated form with 14-3-3 protein. In response to cAMP levels and glucagon, relocated to the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 200186 Human
- Entrez Gene: 310615 Rat
- Omim: 608972 Human
- SwissProt: Q53ET0 Human
- SwissProt: Q3LRZ1 Rat
- Unigene: 406392 Human
- Unigene: 13599 Rat
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Alternative names
- CREB regulated transcription coactivator 2 antibody
- CREB-regulated transcription coactivator 2 antibody
- CRTC2 antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed paraffin-embedded rat lung tissue, labeling TORC2 (phospho S171) using ab203187 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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All lanes : Anti-TORC2 (phospho S171) antibody (ab203187) at 1/200 dilution
Lane 1 : Human rectal carcinoma lysate
Lane 2 : Human gastric carcinoma lysate
Developed using the ECL technique.
Predicted band size: 73 kDa
Observed band size: 73 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab203187 has been referenced in 3 publications.
- Chen X et al. Adropin regulates hepatic glucose production via PP2A/AMPK pathway in insulin-resistant hepatocytes. FASEB J 34:10056-10072 (2020). PubMed: 32579277
- Song D et al. Adenovirus-mediated expression of SIK1 improves hepatic glucose and lipid metabolism in type 2 diabetes mellitus rats. PLoS One 14:e0210930 (2019). PubMed: 31233505
- Li P et al. CRTC2 Is a Key Mediator of Amino Acid-Induced Milk Fat Synthesis in Mammary Epithelial Cells. J Agric Food Chem 67:10513-10520 (2019). PubMed: 31475823