Key features and details
- Rabbit polyclonal to TORC2 (phospho S171)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-TORC2 (phospho S171) antibody
See all TORC2 primary antibodies
DescriptionRabbit polyclonal to TORC2 (phospho S171)
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
- Human rectal carcinoma and gastric carcinoma lysates. Rat lung tissue.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 0.01% BSA
Concentration information loading...
PurityProtein A purified
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
Our Abpromise guarantee covers the use of ab203187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at 1/50 - 1/200 with fluorescent detection methods.
FunctionTranscriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).
Tissue specificityMost abundantly expressed in the thymus. Present in both B and T lymphocytes. Highly expressed in HEK293T cells and in insulinomas. High levels also in spleen, ovary, muscle and lung, with highest levels in muscle. Lower levels found in brain, colon, heart, kidney, prostate, small intestine and stomach. Weak expression in liver and pancreas.
Sequence similaritiesBelongs to the TORC family.
modificationsPhosphorylation/dephosphorylation states of Ser-171 are required for regulating transduction of CREB activity. TORCs are inactive when phosphorylated, and active when dephosphorylated at this site. This primary site of phosphorylation, is regulated by cAMP and calcium levels and is dependent on the phosphorylation of SIKs by LKB1. Both insulin and AMPK increase this phosphorylation, of TORC2 while glucagon suppresses it.
Cellular localizationCytoplasm. Nucleus. Translocated from the nucleus to the cytoplasm on interaction of the phosphorylated form with 14-3-3 protein. In response to cAMP levels and glucagon, relocated to the nucleus.
- Information by UniProt
- CREB regulated transcription coactivator 2 antibody
- CREB-regulated transcription coactivator 2 antibody
- CRTC2 antibody
Immunohistochemical analysis of formalin-fixed paraffin-embedded rat lung tissue, labeling TORC2 (phospho S171) using ab203187 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
All lanes : Anti-TORC2 (phospho S171) antibody (ab203187) at 1/200 dilution
Lane 1 : Human rectal carcinoma lysate
Lane 2 : Human gastric carcinoma lysate
Developed using the ECL technique.
Predicted band size: 73 kDa
Observed band size: 73 kDa
ab203187 has been referenced in 1 publication.
- Song D et al. Adenovirus-mediated expression of SIK1 improves hepatic glucose and lipid metabolism in type 2 diabetes mellitus rats. PLoS One 14:e0210930 (2019). PubMed: 31233505