Overview

  • Product name

    Anti-TORC2 (phospho S171) antibody
    See all TORC2 primary antibodies
  • Description

    Rabbit polyclonal to TORC2 (phospho S171)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human TORC2 aa 150-190 (phospho S171) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Sequence:

    G NFPAEKGQLF RLPSALNRTS SDSALHTSVM NPSPQDTYPG


    Database link: Q53ET0

  • Positive control

    • Human rectal carcinoma and gastric carcinoma lysates. Rat lung tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab203187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Use at 1/50 - 1/200 with fluorescent detection methods.

Target

  • Function

    Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).
  • Tissue specificity

    Most abundantly expressed in the thymus. Present in both B and T lymphocytes. Highly expressed in HEK293T cells and in insulinomas. High levels also in spleen, ovary, muscle and lung, with highest levels in muscle. Lower levels found in brain, colon, heart, kidney, prostate, small intestine and stomach. Weak expression in liver and pancreas.
  • Sequence similarities

    Belongs to the TORC family.
  • Post-translational
    modifications

    Phosphorylation/dephosphorylation states of Ser-171 are required for regulating transduction of CREB activity. TORCs are inactive when phosphorylated, and active when dephosphorylated at this site. This primary site of phosphorylation, is regulated by cAMP and calcium levels and is dependent on the phosphorylation of SIKs by LKB1. Both insulin and AMPK increase this phosphorylation, of TORC2 while glucagon suppresses it.
  • Cellular localization

    Cytoplasm. Nucleus. Translocated from the nucleus to the cytoplasm on interaction of the phosphorylated form with 14-3-3 protein. In response to cAMP levels and glucagon, relocated to the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CREB regulated transcription coactivator 2 antibody
    • CREB-regulated transcription coactivator 2 antibody
    • CRTC2 antibody
    • CRTC2_HUMAN antibody
    • RP11-422P24.6 antibody
    • TORC-2 antibody
    • torc2 antibody
    • Transducer of CREB protein 2 antibody
    • Transducer of regulated cAMP response element-binding protein antibody
    • Transducer of regulated cAMP response element-binding protein (CREB) 2 antibody
    • Transducer of regulated cAMP response element-binding protein 2 antibody
    • Transducer of regulated CREB protein 2 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded rat lung tissue, labeling TORC2 (phospho S171) using ab203187 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

  • All lanes : Anti-TORC2 (phospho S171) antibody (ab203187) at 1/200 dilution

    Lane 1 : Human rectal carcinoma lysate
    Lane 2 : Human gastric carcinoma lysate

    Developed using the ECL technique.

    Predicted band size: 73 kDa
    Observed band size: 73 kDa

References

ab203187 has not yet been referenced specifically in any publications.

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