Anti-Torsin A/DYT1 antibody (ab34540)
Key features and details
- Rabbit polyclonal to Torsin A/DYT1
- Suitable for: IHC-FoFr, ICC/IF, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Torsin A/DYT1 antibody
See all Torsin A/DYT1 primary antibodies -
Description
Rabbit polyclonal to Torsin A/DYT1 -
Host species
Rabbit -
Specificity
The immunogen used to raise this antibody has 92% homology with the human Torsin A/DYT1 protein. Some customers have successfully used ab34540 in human tissue. Please contact Abcam Scientific Support for more information.
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Tested applications
Suitable for: IHC-FoFr, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human -
Immunogen
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Positive control
- ICC/IF: Neuro2a cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab34540 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-FoFr | (1) |
Use at an assay dependent concentration.
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ICC/IF |
Use a concentration of 1 µg/ml.
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
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Notes |
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IHC-FoFr
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa). |
Target
- Information by UniProt
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Database links
- Entrez Gene: 1861 Human
- Entrez Gene: 30931 Mouse
- Entrez Gene: 266606 Rat
- Omim: 605204 Human
- SwissProt: O14656 Human
- SwissProt: Q9ER39 Mouse
- Unigene: 534312 Human
- Unigene: 154994 Mouse
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Alternative names
- DQ2 antibody
- Dystonia 1 antibody
- Dystonia 1 protein antibody
see all
Images
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ab34540 staining Torsin A/DYT1 in Neuro2a cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab34540 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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All lanes : Anti-Torsin A/DYT1 antibody (ab34540) at 1 µg/ml
Lane 1 : Marker
Lane 2 : Mouse Brain at 10 µg
Lane 3 : Testis (Mouse) Whole Cell Lysate - normal tissue at 10 µg
Lane 4 : Mouse Heart Mouse Tissue Lysate at 10 µg
Lane 5 :Mouse skeletal muscle tissue lysate - total protein (ab29711) at 10 µg
Lane 6 : Mouse Fetal lysate - 14 Days old Tissue Lysate at 10 µg
Lane 7 :Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg
Lane 8 : Mouse Embryonic Fibroblast 1 at 10 µg
Lane 9 :NIH/3T3 whole cell lysate (ab7179) at 10 µg
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 38 kDa
Additional bands at: 105 kDa, 65 kDa. We are unsure as to the identity of these extra bands. -
All lanes : Anti-Torsin A/DYT1 antibody (ab34540) at 1 µg/ml
Lane 1 :NIH/3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 5 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 38 kDa
Additional bands at: 65 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (32)
ab34540 has been referenced in 32 publications.
- Ponterio G et al. Alpha-Synuclein is Involved in DYT1 Dystonia Striatal Synaptic Dysfunction. Mov Disord 37:949-961 (2022). PubMed: 35420219
- Yokoi F et al. Characterization of the direct pathway in Dyt1 ?GAG heterozygous knock-in mice and dopamine receptor 1-expressing-cell-specific Dyt1 conditional knockout mice. Behav Brain Res 411:113381 (2021). PubMed: 34038798
- Scuteri D et al. Effect of Gabapentin in a Neuropathic Pain Model in Mice Overexpressing Human Wild-Type or Human Mutated Torsin A. Life (Basel) 11:N/A (2021). PubMed: 33445430
- Li J et al. TorsinA restoration in a mouse model identifies a critical therapeutic window for DYT1 dystonia. J Clin Invest 131:N/A (2021). PubMed: 33529159
- Yokoi F et al. Decreased number of striatal cholinergic interneurons and motor deficits in dopamine receptor 2-expressing-cell-specific Dyt1 conditional knockout mice. Neurobiol Dis 134:104638 (2020). WB ; Mouse . PubMed: 31618684