Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2569Y] to Torsin A/DYT1
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Product nameAnti-Torsin A/DYT1 antibody [EP2569Y]
See all Torsin A/DYT1 primary antibodies
DescriptionRabbit monoclonal [EP2569Y] to Torsin A/DYT1
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Torsin A/DYT1 aa 300-400 (C terminal). The exact sequence is proprietary.
- WB: SW480, Caco-2, A549, NIH/3T3, and C6 whole cell lysates. Rat brain and mouse lung tissue lysates; Flow Cyt: SH-SY5Y cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as Torsin A
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, 59% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab76133 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa).|
For unpurified used at 1/150
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
- Information by UniProt
- DQ2 antibody
- Dystonia 1 antibody
- Dystonia 1 protein antibody
All lanes : Anti-Torsin A/DYT1 antibody [EP2569Y] (ab76133) at 1/5000 dilution (purified)
Lane 1 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Rat brain lysate
Lane 4 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 6 : Mouse lung lysate
Lane 7 : C6 (Rat glial tumor glial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Torsin A/DYT1 with purified ab76133 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
ab76133 has not yet been referenced specifically in any publications.