Key features and details
- Mouse polyclonal to TOSO
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TOSO antibody
See all TOSO primary antibodies
DescriptionMouse polyclonal to TOSO
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human TOSO aa 1-390.
MDFWLWPLYFLPVSGALRILPEVKVEGELGGSVTIKCPLPEMHVRIYLCR EMAGSGTCGTVVSTTNFIKAEYKGRVTLKQYPRKNLFLVEVTQLTESDSG VYACGAGMNTDRGKTQKVTLNVHSEYEPSWEEQPMPETPKWFHLPYLFQM PAYASSSKFVTRVTTPAQRGKVPPVHHSSPTTQITHRPRVSRASSVAGDK PRTFLPSTTASKISALEGLLKPQTPSYNHHTRLHRQRALDYGSQSGREGQ GFHILIPTILGLFLLALLGLVVKRAVERRKALSRRARRLAVRMRALESSQ RPRGSPRPRSQNNIYSACPRRARGADAAGTGEAPVPGPGAPLPPAPLQVS ESPWLHAPSLKTSCEYVSLYHQPAAMMEDSDSDDYINVPA
Database link: NP_005440.1
- Human tonsil tissue; TOSO transfected 293T cell lysate.
This product was previously labelled as FAIM3
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.4
Constituent: 100% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab168968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa.|
|IHC-P||Use a concentration of 3 µg/ml.|
FunctionMay play a role in the immune system processes. Protects cells from FAS-, TNF alpha- and FADD-induced apoptosis without increasing expression of the inhibitors of apoptosis BCL2 and BCLXL. Seems to activate an inhibitory pathway that prevents CASP8 activation following FAS stimulation, rather than blocking apoptotic signals downstream. May inhibit FAS-induced apoptosis by preventing CASP8 processing through CFLAR up-regulation.
Tissue specificityExpressed in lymph nodes, peripheral blood leukocytes, lung, thymus and kidneys. Very weak expression detected in spleen, liver, heart, and salivary gland. Expressed in lymphoid cell lines such as Jurkat cells, CemT4 cells, MolT-4 cells, HB11.19 cells, and Reh cells. No expression detected in nonhematopoietic cell lines, including HepG2 cells, 293 cells and Hela cells.
Sequence similaritiesContains 1 Ig-like (immunoglobulin-like) domain.
DomainThe Ig-like domain is required for the anti-apoptotic ability.
- Information by UniProt
- Faim3 antibody
- FAIM3_HUMAN antibody
- Fas apoptotic inhibitory molecule 3 antibody
All lanes : Anti-TOSO antibody (ab168968) at 1 µg/ml
Lane 1 : TOSO transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 43 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling TOSO with ab168968 at 3 μg/ml.
ab168968 has not yet been referenced specifically in any publications.