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Inquiry: Dear sir/madam I'm going to measure total antioxidant capacity intracellular mouse spermatozoa, Is kit ab65329 appropriate for this measurment? if your answer is YES, how many mouse spermatozoa is needed for this measurment? if your answer is NO, do you have any kit for this measurment? I'm looking forward to hearing from you as soon as possible. Best regards
Asked on Dec 07 2012
Thank you for your enquiry.
I am sorry to confirm we have not specifically tested mouse spermatozoa using this kit. But since the kit is compatible with cells of murine origin, I do not see why it would not work.
We would recommend to start with a relatively large number of cells, ˜ 2 - 5 x 10^7 cells. Lyse them in the assay diluent and use different amounts for the assay to find the optimum.
Hope this information has been useful for you. Please do not hesitate to let me know if you have any other questions.
Answered on Dec 07 2012