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Can the kit measure the antioxidant activity of an extract of a plant? If Yes, may i know the method to measure... waiting for your reply... with thanks.
Asked on Feb 26 2013
This kit has only been optimized for mammalian sample. Theoretically, it could be used in samples of multiple species including plant. Because we have not tested it with any plant sample, I am sorry that there is no specific protocol for measuring plant sample.
In order to get the most efficient result, you may have to optimize the original protocol.
Original protocol for preparation of tissue sample:
For Tissue Samples, Start with 10-100 mg of the tissue, add 500-1000 μl (or ˜4-6 volumes) of the PBS on ice, homogenize using a Dounce homogenizer (10-50 passes) on ice, until efficient lysis is confirmed, by viewing the cells under the microscope. Sonication can also be performed instead. Spin down the sample at 10000 rpm for 10 min at 4°C and collect the supernatant. Use the supernatant for the subsequent assays.
Answered on Feb 26 2013