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Dear Abcam Scientific Support Team,
I would like to check the total antioxidant capacity of the plasma samples that contain EDTA as an anticoagulant. Does the EDTA interfere with the ab65329 TAC assay?
Asked on Mar 04 2013
Thank you for contacting us.
Although I don’t think EDTA will have an adverse effect on the efficiency of this kit, I would not recommend using EDTA for plasma preparations. Instead use heparin or citrate.
EDTA anticoagulant will give a lower antioxidant capacity compared with heparinized plasma samples but nonetheless, sufficient for detection and data analysis.
I have included a publication mentioning the difference between EDTA and heparin anticoagulants for your reference.
The effect of anticoagulant choice on apparent total antioxidant capacity using three different methods. Ann Clin Biochem. 1995 Jul;32 ( Pt 4):413-6. http://www.ncbi.nlm.nih.gov/pubmed/7486802
I hope this information is helpful. Please do not hesitate to contact us if you require further assistance.
Answered on Mar 04 2013