Overview

  • Product name

    Total Carbohydrate Assay Kit - Quantification
  • Detection method

    Colorimetric
  • Sample type

    Tissue, Adherent cells, Suspension cells, Tissue Extracts
  • Assay type

    Quantitative
  • Assay time

    0h 30m
  • Product overview

    Total Carbohydrate Assay (ab155891) is a simple, sensitive and robust method of detecting virtually all carbohydrates.


    The carbohydrate assay protocol is based on the phenol - sulfuric acid assay. In the assay, polysaccharides (mono, di, tri, etc.) and their derivatives, in the presence of sulfuric acid, are hydrolyzed to monomers and converted to furfural or hydroxyfurfural. These react with the developer to form a chromogen that can be quantified by measuring the absorbance at λ = 490 nm.


    The total carbohydrate assay kit can detect most forms of carbohydrates, including simple and complex saccharides, glycans, glycoproteins and glycolipids.


    Total carbohydrate assay protocol summary:
    - add samples and glucose standards to wells
    - add concentrated sulfuric acid and incubate for 15 min at 90ºC
    - add developer and incubate for 5 min
    - analyze with a microplate reader

  • Notes

    Carbohydrates play important structural as well as chemical roles in all living systems. Detection of total carbohydrates, therefore, has wide applications.

  • Platform

    Microplate reader

Properties

Images

  • Carbohydrates in food items and mouse tissue lysates, shown as mg per mg of extracted protein (duplicates; +/- SD).

  • Carbohydrates measured in orange juice and caster sugar. Background signal subtracted, duplicates; +/- SD.

  • Carbohydrates from lysed non-differentiated or adipose differentiated 3T3L1 cells (duplicates; +/- SD). Cells were differentiated using a mixture of small molecules: ab120840, ab120743 and ab123768.

  • D-Glucose standard curve generated using the method described in the protocol bookelt. 

Protocols

References

ab155891 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

We have not tested ab155891 with CMC. The sulphuric acid hydrolysis might need to be optimized for CMC. 15 mins may not be enough in this case.

Read More

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