Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Adherent cells, Suspension cells, Tissue, Tissue Extracts
Product nameTotal Carbohydrate Assay Kit - Quantification
Sample typeTissue, Adherent cells, Suspension cells, Tissue Extracts
Assay time0h 30m
Total Carbohydrate Assay (ab155891) is a simple, sensitive and robust method of detecting virtually all carbohydrates.
The carbohydrate assay protocol is based on the phenol - sulfuric acid assay. In the assay, polysaccharides (mono, di, tri, etc.) and their derivatives, in the presence of sulfuric acid, are hydrolyzed to monomers and converted to furfural or hydroxyfurfural. These react with the developer to form a chromogen that can be quantified by measuring the absorbance at λ = 490 nm.
The total carbohydrate assay kit can detect most forms of carbohydrates, including simple and complex saccharides, glycans, glycoproteins and glycolipids.
Total carbohydrate assay protocol summary:
- add samples and glucose standards to wells
- add concentrated sulfuric acid and incubate for 15 min at 90ºC
- add developer and incubate for 5 min
- analyze with a microplate reader
Carbohydrates play important structural as well as chemical roles in all living systems. Detection of total carbohydrates, therefore, has wide applications.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Carbohydrates in food items and mouse tissue lysates, shown as mg per mg of extracted protein (duplicates; +/- SD).
Carbohydrates measured in orange juice and caster sugar. Background signal subtracted, duplicates; +/- SD.
D-Glucose standard curve generated using the method described in the protocol bookelt.
ab155891 has been referenced in 1 publication.
- Rawashdeh RY et al. Biological interaction levels of zinc oxide nanoparticles; lettuce seeds as case study. Heliyon 6:e03983 (2020). PubMed: 32509982