Overview

  • Product name

    Total Choline Assay Kit
  • Detection method

    Fluorescent
  • Sample type

    Urine, Serum, Plasma, Other biological fluids, Cell Lysate, Tissue Lysate
  • Assay type

    Quantitative
  • Sensitivity

    40 nM
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    Total Choline Detection Kit (ab219944) provides a sensitive fluorescence-based assay for quantifying choline in cell and tissue lysates and biological fluids. The amount of choline is proportional to the concentration of hydrogen peroxide formed in the choline oxidase-mediated enzyme coupling reaction cycle, which in turn is detected with our proprietary Red Indicator. The increase in fluorescence can be easily detected at an optimal Ex/Em = 540/590 nm. The signal can also be read by absorbance at OD: 576 ± 5 nm, although the sensitivity of the assay is reduced 10-fold.


    The assay detects as little as 4 picomole choline in 100 µL assay volume (40 nM), making it one of the most sensitive assays available in the market.


    This product was previously known as Total Choline Detection Kit.

  • Notes

    Choline is an essential nutrient. Choline and its metabolites play an important role in the structural integrity and signaling of cell membranes and cholinergic neurotransmission (choline synthesis). It is a major source of methyl group via its metabolite, trimethylglycine that participates in the S-adenosylmethionine synthesis pathways. Choline deficiency may cause liver disease, atherosclerosis and possibly neurological disorders. Despite its importance in the central nervous system as a precursor for acetylcholine and membrane phosphatidylcholine, the role of choline in mental illness has been little studied.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 200 tests
    Assay Buffer 1 x 25ml
    Choline Probe 2 vials
    Choline Standard (2.8 mg) 1 x 2.8mg
    DMSO 1 x 100µl
    Red Dye 1 vial

Images

  • Typical Choline standard calibration curve. Choline dose response was measured on a 96 well solid black plate using a Gemini fluorescence microplate reader (Molecular Devices). As low as 40nM (4 picomole/well) of choline can be detected with 30 minutes incubation time (n=3).

Protocols

References

ab219944 has not yet been referenced specifically in any publications.

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