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    total-iron-binding-capacity-tibc-and-serum-iron-assay-kit-ab239715.pdf

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Cardiovascular Blood Serum Proteins
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Total Iron-Binding Capacity (TIBC) and Serum Iron Assay Kit (ab239715)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (3) Submit a question References (1)

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Iron Standard Curve
  • Serum Iron and TIBC determination of serum.

Key features and details

  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Sample type: Plasma, Serum

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Overview

  • Product name

    Total Iron-Binding Capacity (TIBC) and Serum Iron Assay Kit
    See all Iron kits
  • Detection method

    Colorimetric
  • Sample type

    Serum, Plasma
  • Product overview

    Total Iron-Binding Capacity (TIBC) and Serum Iron Assay Kit (ab239715) provides a simple, sensitive, and high-throughput adaptable approach to detect physiological Total iron-binding capacity (TIBC) and Serum iron. Those values indicate the requisite iron for transferrin saturation and Serum Iron respectively. Those measurements can be used to detect and monitor transferrin saturation and also iron-deficiency anemia and chronic inflammatory diseases.

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K392 Total Iron-Binding Capacity (TIBC) and Serum Iron Assay Kit (Colorimetric). K392-100 is the same size as the 100 test size of ab239715.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests 2000 tests
    Iron Solution 1 x 100µl 20 x 100µl
    Iron Standard (100 mM) 1 x 100µl 20 x 100µl
    TIBC Assay Buffer 1 x 25ml 20 x 25ml
    TIBC Detector 2 x 1.5ml 40 x 1.5ml
    TIBC Developer 1 x 5ml 20 x 5ml
  • Research areas

    • Cardiovascular
    • Blood
    • Serum Proteins
    • Signal Transduction
    • Metabolism
    • Vitamins / Minerals
    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Other Metabolism Assay
  • Relevance

    Iron is essential to nearly all known organisms. It is generally stored in the centre of metalloproteins, in the heme complex, and in oxygen carrier proteins. Inorganic iron also contributes to redox reactions in the iron-sulfur clusters of many enzymes, such as nitrogenase and hydrogenase.
  • Alternative names

    • Fe
    • Fe++
    • Fe+++

Images

  • Iron Standard Curve
    Iron Standard Curve

    Iron standard Curve generated using the kit protocol.

  • Serum Iron and TIBC determination of serum.
    Serum Iron and TIBC determination of serum.

    Serum Iron and TIBC determination of serum. Assays were performed following the kit protocol.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab239715? Please let us know so that we can cite the reference in this datasheet.

ab239715 has been referenced in 1 publication.

  • Tang X  et al. Transferrin plays a central role in coagulation balance by interacting with clotting factors. Cell Res 30:119-132 (2020). PubMed: 31811276

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-3 of 3 Abreviews or Q&A

Large variability between assay

Below average Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
I used this kit to measure serum iron and TIBC. Each sample must be assayed in 8 wells, thus I could not test more than 10 samples in a 96 well plate (or 20 samples without assaying in duplicate), and I experienced large variation between the experiments (different plates).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 18 2022

Cell culture supernatant iron quantification

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
I was able to quantify iron and the iron binding capacity of compounds in cell culture supernatant.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Charlotte Rigauts

Verified customer

Submitted Aug 17 2021

Not Useable for standard CSF samples

Good Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
I attepmted to use the kit to determine TIBC of cerebrospinal fluid. While the kit worked technically, its detection limit for iron was not sensitive enough. The concentration of iron in CSF is around 6ng/mL. Thus, I would not recommend it for use of CSF samples unless the same have higher than normal amounts of iron.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MRS. Becky Webb

Verified customer

Submitted May 17 2021

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