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I want to inquire about the Total NAD and NADH assay kit (cat# ab18603). Can it be used to measure the levels of NAD and NADH in serum samples? If yes, will I have to use a modified protocol? Thank you.
Asked on Jan 24 2017
I contacted the originating laboratories. They say this kit has been tested externally for plasma and serum samples, but they recommend the following sample preparation protocols:
Serum: Collect blood without anticoagulant. Allow blood to clot for 30 minutes RT. Centrifuge at 2000 x g and 4ºC for 10 min. Remove serum layer and store on ice. Take care to avoid disturbing the white buffy layer. Aliquot samples for testing and store remaining solution at -80ºC. Prior to testing, filter samples with 3K-10K centrifugal filter. Perform serum dilutions in Assay Buffer.
Plasma: Collect blood with heparin or citrate and centrifuge at 1000 x g and 4ºC for 10 minutes. Remove the plasma layer and store on ice. Take care to avoid disturbing the white buffy layer. Aliquot samples for testing and store remaining solution at -80ºC. Perform plasma dilutions in Assay Buffer. Perform serum dilutions in Assay Buffer.
Answered on Jan 24 2017