Overview

  • Product name

    Total Polyamine Assay Kit
  • Detection method

    Fluorescent
  • Sample type

    Other biological fluids, Tissue
  • Product overview

    Total Polyamine Assay Kit (ab239728) enables the rapid determination of polyamine concentration in biological samples. A selective enzyme mix acts on polyamines, generating hydrogen peroxide that is then reacted with a fluorometric probe (Ex/Em = 535/587 nm) to yield a signal proportional to the amount of polyamine present. The kit includes a proprietary Sample Clean-Up reagent for pre-treating samples in order to eliminate common metabolites found in biological samples that may interfere with the assay or increase sample background. The assay is rapid, simple, and high throughput compatible, and can detect polyamine concentrations as low as 0.1 μM in tissue lysates and other samples such as saliva.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Polyamine Assay Buffer 1 x 25ml
    Polyamine Developer 1 vial
    Polyamine Enzyme Mix 1 vial
    Polyamine Probe (in DMSO) 1 x 200µl
    Polyamine Standard 1 vial
    Sample Clean-Up Mix 1 vial
  • Relevance

    Polyamines are small organic molecules bearing two or more primary amine moieties. Biogenic polyamines such as spermidine, putrescine and spermine function as transcellular signaling molecules and are involved in diverse biological processes. Although small amounts of polyamines are synthesized in cells, larger quantities are often encountered as a result of putrefaction and decay, as they are the direct product of decarboxylation of amino acids such as methionine, lysine and arginine. The enzyme ornithine decarboxylase generates the polyamine putrescine, which is not only responsible for repulsive odor but is also implicated in cancer. Intracellular polyamines readily bind DNA and are critical to preventing oxidative DNA damage and directing DNA double-strand break repair pathways. Polyamine levels decline with age and dietary supplementation of the polyamine spermidine has recently been shown to reduce age-related oxidative stress and extend lifespan in mouse models of aging.

Images

  • Determination of total polyamine concentration in saliva (determined to be 43.0 +/- 8.9 µM) and intestinal tissue lysate (63.9 +/- 16.0 nmole/mg protein). For this experiment, 100 mg rat intestine was homogenized and prepared according to the kit protocol. Saliva (2 µL) and intestinal lysate were treated with Sample Clean-Up Mix. Values are mean ± standard deviation of at least three independent determinations.

Protocols

References

ab239728 has not yet been referenced specifically in any publications.

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