Overview

  • Product name

    Total Sulphite Assay Kit (Colorimetric)
  • Detection method

    Colorimetric
  • Sample type

    Food samples
  • Assay type

    Quantitative
  • Sensitivity

    20 µM
  • Product overview

    Total Sulphite Assay Kit (Colorimetric) (ab211102) provides a simple and sensitive assay to detect small concentrations of sulphite present in a variety of food and beverage samples such as wine, juices, dairy, canned products, juices or dried fruit. This assay is based on the oxidation of sulphite to sulfate, which reacts with the enzyme mix to produce a stable signal that can be detected at OD = 570 nm, and that is directly proportional to the amount of sulphite present in the sample. This assay is very sensitive and can detect as low as 20 µM of sulphite in a variety of samples.

  • Notes

    Sulphites (also known as sulfites) are substances that occur naturally in the human body as by-products of the metabolism of amino acids containing sulfur side chains. They are readily oxidized to sulfates via several enzymatic reactions and excreted in urine at the rate of 1000 mg/day. The term sulphite is used for all sulphite-derived molecules: bisulphites, metasulphites, and SO2. Total sulphite is defined as the sum of bound and free sulphite.

    Sulphites are often considered as allergens due to the adverse symptoms observed in asthmatic patients. Exogenous sources of sulphites include polluted air, and food and beverages containing sulphur dioxide (SO2). SO2 is a molecule that easily reacts with several small compounds including aldehydes, ketones, anthocyanins, cobalamine, thiamine, NAD and flavins. SO2 also interacts with cysteine residues in proteins and promotes cross-linking. Sulphites are also used as regulated food preservatives/additives/enhancers in dried fruits, wine and beer. They are considered as GRAS (Generally Recognized as Safe) in food and beverages. In wine, SO2 reacts with sugars, aldehydes and anthocyanins.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Sulphite Assay Buffer 1 x 25ml
    Sulphite Enzyme Mix (200 µg) 1 vial
    Sulphite Oxidizing Mix 1 x 80µl
    Sulphite Probe 1 x 200µl
    Sulphite Stabilizer 1 x 25ml
    Sulphite Standard (200 µmol) 5 vials

Associated products

Images

  • Typical Sulphite standard calibration curve.

  • Estimation of Total Sulphite Concentration in two wine samples. White wine was diluted 16-fold in Sulphite Assay Buffer and 25 µL of diluted sample was spiked with 6 nmol of Sulphite Standard. Red wine was neutralized, clarified using PVPP, and spiked with 6 nmol of Sulphite Standardl. Samples were assayed following the kit protocol. Total Sulphite concentrations: Red Wine: 24.2 mg/L SO2; White wine: 80.9 mg/L SO2.

Protocols

References

ab211102 has not yet been referenced specifically in any publications.

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