Overview

  • Product name

    Anti-TOX3 antibody [TOX3/1123]
    See all TOX3 primary antibodies
  • Description

    Mouse monoclonal [TOX3/1123] to TOX3
  • Host species

    Mouse
  • Tested applications

    Suitable for: Protein Array, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human TOX3 aa 200-400. The exact sequence is proprietary. (139 amino acid fragment).
    Database link: O15405

  • Positive control

    • Human gastric carcinoma, gall bladder and cervical carcinoma tissues.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    ab216614 was purified from Bioreactor Concentrate by Protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    TOX3/1123
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab216614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Protein Array Use at an assay dependent concentration.
IHC-P Use a concentration of 0.5 - 1 µg/ml.

Perform heat mediated antigen retrieval with Tris/EDTA pH 9 or citrate buffer pH 6 before commencing with IHC staining protocol. (Primary incubation for 30 minutes at RT).

Target

Images

  • ab216614 was tested in protein array against over 19000 different full-length human proteins.
    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
    A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human gastric carcinoma tissue labeling TOX3 with ab216614 at 1 μg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human gall blasser tissue labeling TOX3 with ab216614 at 1 μg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cervical carcinoma tissue labeling TOX3 with ab216614 at 1 μg/ml.

References

ab216614 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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