Anti-TOX3 antibody [TOX3/1123] - BSA and Azide free (ab212414)
Key features and details
- Mouse monoclonal [TOX3/1123] to TOX3 - BSA and Azide free
- Suitable for: Protein Array, IHC-P
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-TOX3 antibody [TOX3/1123] - BSA and Azide free
See all TOX3 primary antibodies -
Description
Mouse monoclonal [TOX3/1123] to TOX3 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: Protein Array, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human TOX3 (internal sequence). The exact sequence is proprietary. 139 amino acid residues around aa 200-400
Database link: O15405 -
Positive control
- SH-SY5Y, MDA-MB-435 cells. Brain or breast or gastric Carcinoma.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
TOX3/1123 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab212414 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Protein Array |
Use at an assay dependent concentration.
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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Protein Array
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 27324 Human
- Omim: 611416 Human
- SwissProt: O15405 Human
- Unigene: 460789 Human
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Alternative names
- BC052044 antibody
- C230068E13 antibody
- CAG trinucleotide repeat-containing gene F9 protein antibody
see all
Images
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This data was produced with ab216614, the same antibody in a different formulation with BSA and Azide.
ab216614 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29. -
Immunohistochemical analysis of formalin fixed paraffin embedded human Cervical carcinoma labeling TOX3 with ab212414 at 1 μg/ml
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Immunohistochemical analysis of formalin fixed paraffin embedded Human Gall bladder labeling TOX3 with ab212414 at 1 μg/ml
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Immunohistochemical analysis of formalin fixed paraffin embedded Human gastric carcinoma labeling TOX3 with ab212414 at 1 μg/ml
Datasheets and documents
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Datasheet download
References (1)
ab212414 has been referenced in 1 publication.
- Yang W et al. TOX3 regulates the proliferation and apoptosis of colorectal cancer by downregulating RhoB via the activation of the MAPK pathway. Cell Biol Int 46:1074-1088 (2022). PubMed: 35347804