Overview

  • Product name

    Anti-Toxoplasma gondii antibody (Biotin)
    See all Toxoplasma gondii primary antibodies
  • Description

    Goat polyclonal to Toxoplasma gondii (Biotin)
  • Host species

    Goat
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: ELISAmore details
  • Species reactivity

    Reacts with: Toxoplasma gondii
  • Immunogen

    RH Strain of Toxoplasma gondii.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.

Target

  • Relevance

    Toxoplasma is a crescent shaped sporozoan that lives as an intracellular parasite in various tissues of many vertebrates and completes its life cycle in a single host. Its life cycle includes two phases called the intestinal (or enteroepithelial) and extraintestinal phases. The intestinal phase produces oocysts and occurs only in cats, wild as well as domesticated. The extraintestinal phase occurs in all infected animals including cats, and produces tachyzoites (actively proliferating trophozoites) and eventually, bradyzoites (slowly growing trophozoites) or zoitocysts. Infection due to Toxoplasma gondii occurs in pregnant women where a variable degree of immunosuppression may exist or in patients receiving immunosuppressive drug therapy. Toxoplasma infects tissue of the GI tract where an active infection is accompanied by fever and enlargement of the spleen. Symptoms of toxoplasmosis are generally mild but severe infection of lymph nodes may occur. Congenital toxoplasmosis, in which the maternal infection is transmitted during pregnancy, can produce blindness or mental retardation in the newborn.
  • Database links

    • Alternative names

      • T. gondii antibody

    References

    ab69251 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for contacting us.

    If you are looking for an antibody to detect Toxoplasma gondii in frozen tissue sections, then the best choice is probably ab15170 or ab23507. Even though neither have been tested on sections of frozen tissue, the IHC data for detection in paraffin-embedded tissue is very clean and specific, for ab15170, and detection in frozen tissue is generally easier than detection in formalin-fixed paraffin-embedded tissue. The alternative, goat polyclonal ab23507, has been published twice.

    Our guarantee does not apply to IHC of frozen tissue using either of these antibodies, but I can offer a testing discount for either, if you are interested. For details, please see the web page at the following URL:

    www.abcam.com/abtrial

    For detection of ab15170, you will need an anti-rabbit IgG secondary antibody conjugated to an enzyme such as HRP, or a fluorochrome such as FITC. For an HRP conjugate, we recommend ab6802:

    Click here (or use the following: https://www.abcam.com/index.html?datasheet=6802).

    For the goat polyclonal ab23507, we recommend the HRP conjugate ab6741:

    Click here (or use the following: https://www.abcam.com/index.html?datasheet=6741).

    For fluorochrome conjugates, please reply and I will help.

    Read More

    Answer

    Thank you for contacting us.

    The secondary antibody ab6741 will probably recognize the biotinylated antibody ab69251 but they have not been tested together. You may want to consider using the streptavidin-HRP conjugate ab7403 instead of a secondary antibody. Or, you may want to order a different primary antibody, without biotin, such as the HRP-conjugated ab21182:

    Click here (or use the following: https://www.abcam.com/index.html?datasheet=21182).

    Click here (or use the following: https://www.abcam.com/index.html?datasheet=7403).

    The Toxoplasma gondii antibody ab69251 should be used at a dilution of 1/1000 but you may need to optimize the dilution factor depending on your results. I recommend diluting the antibody in Tris-buffered saline, pH 7.4 for detection. The secondary ab6741 can be applied very dilute at 1/75,000 but again, this may need to optimized since these two antibodies have not been used together before.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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