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1- I will select a Primary antibody [(Anti-Toxoplasma gondii antibody (Biotin) (ab69251)], I wonder if the secondary antibody that I most to choose [(Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab6741)] is work with the primary antibody [(Anti-Toxoplasma gondii antibody (Biotin) (ab69251)].
2- Would you please advise me which kind of buffer and the dilution factor I can use to dilute the primary as well as the secondary antibodies for ELISA test.
Asked on Aug 15 2012
Thank you for contacting us.
The secondary antibody ab6741 will probably recognize the biotinylated antibody ab69251 but they have not been tested together. You may want to consider using the streptavidin-HRP conjugate ab7403 instead of a secondary antibody. Or, you may want to order a different primary antibody, without biotin, such as the HRP-conjugated ab21182:
Click here (or use the following: https://www.abcam.com/index.html?datasheet=21182).
Click here (or use the following: https://www.abcam.com/index.html?datasheet=7403).
The Toxoplasma gondii antibody ab69251 should be used at a dilution of 1/1000 but you may need to optimize the dilution factor depending on your results. I recommend diluting the antibody in Tris-buffered saline, pH 7.4 for detection. The secondary ab6741 can be applied very dilute at 1/75,000 but again, this may need to optimized since these two antibodies have not been used together before.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Aug 15 2012