Overview

  • Product name

    Anti-Toxoplasma gondii antibody [TP3]
    See all Toxoplasma gondii primary antibodies
  • Description

    Mouse monoclonal [TP3] to Toxoplasma gondii
  • Host species

    Mouse
  • Specificity

    This antibody reacts with p30 membrane protein in ELISA and immunoblotting
  • Tested applications

    Suitable for: ELISA, ICC/IF, WBmore details
  • Immunogen

    Tachizoites lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    PBS with 0.1% sodium azide, pH 7.4
  • Concentration information loading...
  • Purity

    Ascites
  • Purification notes

    Purified from ascites.
  • Clonality

    Monoclonal
  • Clone number

    TP3
  • Myeloma

    Sp2/0
  • Isotype

    IgG2a
  • Light chain type

    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

Target

  • Relevance

    Toxoplasma is a crescent shaped sporozoan that lives as an intracellular parasite in various tissues of many vertebrates and completes its life cycle in a single host. Its life cycle includes two phases called the intestinal (or enteroepithelial) and extraintestinal phases. The intestinal phase produces oocysts and occurs only in cats, wild as well as domesticated. The extraintestinal phase occurs in all infected animals including cats, and produces tachyzoites (actively proliferating trophozoites) and eventually, bradyzoites (slowly growing trophozoites) or zoitocysts. Infection due to Toxoplasma gondii occurs in pregnant women where a variable degree of immunosuppression may exist or in patients receiving immunosuppressive drug therapy. Toxoplasma infects tissue of the GI tract where an active infection is accompanied by fever and enlargement of the spleen. Symptoms of toxoplasmosis are generally mild but severe infection of lymph nodes may occur. Congenital toxoplasmosis, in which the maternal infection is transmitted during pregnancy, can produce blindness or mental retardation in the newborn.
  • Alternative names

    • T. gondii antibody

References

This product has been referenced in:

  • Majumdar T  et al. Tryptophan-kynurenine pathway attenuates ß-catenin-dependent pro-parasitic role of STING-TICAM2-IRF3-IDO1 signalosome in Toxoplasma gondii infection. Cell Death Dis 10:161 (2019). Read more (PubMed: 30770800) »
  • Rajendran E  et al. Cationic amino acid transporters play key roles in the survival and transmission of apicomplexan parasites. Nat Commun 8:14455 (2017). IF . Read more (PubMed: 28205520) »
See all 5 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your enquiry. To use this antibody in IF I would recommend starting at a dilution of 1:100 or 1:200. Your customer will have to optimize based upon their results. Also, I updated our online datasheet to include the concentration of the current batch, 164861, that we have in stock; the concentration of this batch is 3.60 mg/ml. Please contact us again if you have any additional questions.

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Answer

Thank you for your enquiry. The antibody TP3 reacts only with p30 band in Western Blotting. It has been tested with purified Toxoplasma antigen and tachyzoites lysate.

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