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Abcam’s anti-Toxoplasma gondii IgA Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgA class antibodies against Toxoplasma gondii in Human serum and plasma.
A 96-well plate has been precoated with Toxoplasma gondii antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgA conjugate is added to the wells, which binds to the immobilized Toxoplasma gondii antigens. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Toxoplasma gondii IgA sample captured in plate.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|Cover Foil||1 unit|
|IgA Sample Diluent||1 x 100ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
|Toxoplasma gondii (IgA) Coated Microplate (12 x 8 wells)||1 unit|
|Toxoplasma gondii Anti-IgA HRP Conjugate||1 x 20ml|
|Toxoplasma gondii IgA Cut-off Control||1 x 3ml|
|Toxoplasma gondii IgA Negative Control||1 x 2ml|
|Toxoplasma gondii IgA Positive Control||1 x 2ml|
Our Abpromise guarantee covers the use of ab178656 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent concentration.|
ab178656 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"