Anti-TP53INP1 antibody (ab9775)
Key features and details
- Rabbit polyclonal to TP53INP1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-TP53INP1 antibody
See all TP53INP1 primary antibodies -
Description
Rabbit polyclonal to TP53INP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human TP53INP1 aa 1-100.
(Peptide available asab9776) -
Positive control
- Human lung, human spleen,tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9775 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 - 2 µg/ml. Can be blocked with Human TP53INP1 peptide (ab9776).
Human lung tissue lysate can be used as a positive control and a band at 27 kDa can be detected. A lower band at 18 kDa was detected in human spleen, tissue lysates. |
IHC-P |
Use a concentration of 5 µg/ml.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Can be blocked with Human TP53INP1 peptide (ab9776). Human lung tissue lysate can be used as a positive control and a band at 27 kDa can be detected. A lower band at 18 kDa was detected in human spleen, tissue lysates. |
IHC-P
Use a concentration of 5 µg/ml. |
Images
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Immunohistochemistry (Formalin-fixed paraffin embedded sections) of human liver tissue labelingp53 DINP1/TP53INP1 with Anti-p53 DINP1/TP53INP1 antibody (ab9775) at 5μg/ml.
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Western blot analysis of p53DINP1 expression in human lung tissue lysate with anti-p53DINP1 (ab9775) at 2
Western blot analysis of p53DINP1 expression in human lung tissue lysate with anti-p53DINP1 (ab9775) at 2 µg/ml in the absence (lane 1) or presence (lane 2) of blocking peptide (ab9776).µ g/ml in the absence (lane 1) or presence (lane 2) of blocking peptide (ab9776).
Protocols
Datasheets and documents
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Datasheet download
References (8)
ab9775 has been referenced in 8 publications.
- Tu Y et al. Long non-coding RNA MEG3 promotes cataractogenesis by upregulating TP53INP1 expression in age-related cataract. Exp Eye Res 199:108185 (2020). PubMed: 32841649
- Fang Y et al. Exosomal miRNA-106b from cancer-associated fibroblast promotes gemcitabine resistance in pancreatic cancer. Exp Cell Res 383:111543 (2019). PubMed: 31374207
- Gao F & Wang W MicroRNA-96 promotes the proliferation of colorectal cancer cells and targets tumor protein p53 inducible nuclear protein 1, forkhead box protein O1 (FOXO1) and FOXO3a. Mol Med Rep 11:1200-6 (2015). PubMed: 25369914
- Zhang J et al. microRNA-155 acts as an oncogene by targeting the tumor protein 53-induced nuclear protein 1 in esophageal squamous cell carcinoma. Int J Clin Exp Pathol 7:602-10 (2014). WB, IHC-P ; Human . PubMed: 24551280
- Wang Y et al. Overexpression of SIRT1 promotes high glucose-attenuated corneal epithelial wound healing via p53 regulation of the IGFBP3/IGF-1R/AKT pathway. Invest Ophthalmol Vis Sci 54:3806-14 (2013). PubMed: 23661372
- Polioudakis D et al. A Myc-microRNA network promotes exit from quiescence by suppressing the interferon response and cell-cycle arrest genes. Nucleic Acids Res 41:2239-54 (2013). WB . PubMed: 23303785
- Zhou Y et al. Survival of pancreatic beta cells is partly controlled by a TCF7L2-p53-p53INP1-dependent pathway. Hum Mol Genet : (2011). WB ; Rat . PubMed: 21965303
- Jiang F et al. MiR-125b promotes proliferation and migration of type II endometrial carcinoma cells through targeting TP53INP1 tumor suppressor in vitro and in vivo. BMC Cancer 11:425 (2011). WB ; Human . PubMed: 21970405