Anti-TPA Tissue Plasminogen Activator antibody [2A153] (ab21049)


  • Product name
    Anti-TPA Tissue Plasminogen Activator antibody [2A153]
    See all TPA Tissue Plasminogen Activator primary antibodies
  • Description
    Mouse monoclonal [2A153] to TPA Tissue Plasminogen Activator
  • Host species
  • Tested applications
    Suitable for: WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Human, Xenopus laevis
  • Immunogen

    Recombinant full length protein: human tPA

  • Positive control
    • Recombinant human TPA
  • General notes

    This product used to be produced in ascites, lots up to and including GR248929-9 were produced in Ascites. All later lots are produced in TCS. 



Our Abpromise guarantee covers the use of ab21049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    IHC-FoFr: Use at an assay dependent dilution (PMID 19074264).
    WB: Use at a concentration of 1 µg/ml. Use under non reducing conditions. Predicted molecular weight: 62 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Play a direct role in facilitating neuronal migration.
    • Tissue specificity
      Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.
    • Involvement in disease
      Note=Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism.
    • Sequence similarities
      Belongs to the peptidase S1 family.
      Contains 1 EGF-like domain.
      Contains 1 fibronectin type-I domain.
      Contains 2 kringle domains.
      Contains 1 peptidase S1 domain.
    • Domain
      Both FN1 and one of the kringle domains are required for binding to fibrin.
      Both FN1 and EGF-like domains are important for binding to LRP1.
      The FN1 domain mediates binding to annexin A2.
      The second kringle domain is implicated in binding to cytokeratin-8 and to the endothelial cell surface binding site.
    • Post-translational
      The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.
      Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin.
      N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor.
      Characterization of O-linked glycan was studied in Bowes melanoma cell line.
    • Cellular localization
      Secreted > extracellular space.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alteplase antibody
      • DKFZp686I03148 antibody
      • Plasminogen activator tissue antibody
      • Plasminogen activator tissue type antibody
      • PLAT antibody
      • Reteplase antibody
      • t PA antibody
      • T Plasminogen Activator antibody
      • t-PA antibody
      • T-plasminogen activator antibody
      • Tissue plasminogen activator (t PA) antibody
      • Tissue type plasminogen activator antibody
      • Tissue-type plasminogen activator chain B antibody
      • tPA antibody
      • TPA_HUMAN antibody
      • TPA1 antibody
      see all


    This product has been referenced in:
    • Bestman JE & Cline HT The RNA binding protein CPEB regulates dendrite morphogenesis and neuronal circuit assembly in vivo. Proc Natl Acad Sci U S A 105:20494-9 (2008). IHC-FoFr ; Xenopus laevis . Read more (PubMed: 19074264) »

    See 1 Publication for this product

    Customer reviews and Q&As

    The exact epitope for Catalog # N63520M has not been mapped at this time, but it recognizes the heavy chain of Tissue Plasminogen Activator (tPA).

    Abcam has not validated the combination of species/application used in this Abreview.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Rabbit Tissue sections (artery, spleen, small intestine, ovary,)
    artery, spleen, small intestine, ovary,
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

    Abcam user community

    Verified customer

    Submitted Jul 22 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Western blot
    Human Cell lysate - whole cell (A549 cell line)
    Loading amount
    10 µg
    A549 cell line
    Gel Running Conditions
    Reduced Denaturing (10% Bis-Tris gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C

    Abcam user community

    Verified customer

    Submitted Aug 15 2008

    Please find below an extract from our protocol on WB (which can be found here for an explanation of non reducing conditions: E) Denaturation and reduction of the protein Antibodies typic...

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    Thank you for your enquiry. Monoclonal Antibody to Human Tissue Plasminogen Activator(tPA), ab21049, was tested with recombinant human TPA in Western blot. I hope this information helps. Please do not hesitate to contact us again if you need a...

    Read More

    Thank you for your enquiry. We have not seen this pattern in Western blot. The two bands you are seeing could be two closely spaced glycosylated forms of the TPA. I hope this information helps. Please do not hesitate to contact us if you need a...

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