Anti-TPC2 antibody (ab111145)
Key features and details
- Goat polyclonal to TPC2
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TPC2 antibody
See all TPC2 primary antibodies -
Description
Goat polyclonal to TPC2 -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Synthetic peptide corresponding to Human TPC2 (C terminal) conjugated to keyhole limpet haemocyanin.
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Positive control
- Human colon and pancreas tissue
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General notes
Protein previously labeled as Two pore calcium channel protein 2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab111145 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
|
IHC-P
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Nicotinic acid adenine dinucleotide phosphate (NAADP) receptor that may function as one of the major voltage-gated Ca(2+) channels (VDCC) across the lysosomal membrane. May be involved in smooth muscle contraction. -
Tissue specificity
Widely expressed. Expressed at high level in liver and kidney. -
Sequence similarities
Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. Two pore calcium channel subfamily. -
Domain
Each of the two internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Lysosome membrane. Only the acidic lysosomal fraction is sensitive to NAADP. - Information by UniProt
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Database links
- Entrez Gene: 219931 Human
- Omim: 612163 Human
- SwissProt: Q8NHX9 Human
- Unigene: 131851 Human
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Alternative names
- SHEP10 antibody
- TPC2 antibody
- TPC2_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPC2 antibody (ab111145)
ab111145, at 3.75 µg/ml, staining TPC2 in formalin fixed, paraffin embedded Human colon tissue by Immunohistochemistry. Followed by biotinylated secondary antibody, alkaline phosphatase streptavidin and chromogen.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPC2 antibody (ab111145)ab111145, at 3.75 µg/ml, staining TPC2 in formalin fixed, paraffin embedded Human pancreas tissue by Immunohistochemistry. Followed by biotinylated secondary antibody, alkaline phosphatase streptavidin and chromogen.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab111145 has not yet been referenced specifically in any publications.