Overview

  • Product name
    Anti-TPC2 antibody
    See all TPC2 primary antibodies
  • Description
    Goat polyclonal to TPC2
  • Host species
    Goat
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human TPC2 (C terminal) conjugated to keyhole limpet haemocyanin.

  • Positive control
    • Human colon and pancreas tissue
  • General notes

    Protein previously labeled as Two pore calcium channel protein 2.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab111145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Nicotinic acid adenine dinucleotide phosphate (NAADP) receptor that may function as one of the major voltage-gated Ca(2+) channels (VDCC) across the lysosomal membrane. May be involved in smooth muscle contraction.
  • Tissue specificity
    Widely expressed. Expressed at high level in liver and kidney.
  • Sequence similarities
    Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. Two pore calcium channel subfamily.
  • Domain
    Each of the two internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Lysosome membrane. Only the acidic lysosomal fraction is sensitive to NAADP.
  • Information by UniProt
  • Database links
    • Alternative names
      • SHEP10 antibody
      • TPC2 antibody
      • TPC2_HUMAN antibody
      • tpcn2 antibody
      • Two pore calcium channel protein 2 antibody
      • two pore segment channel 2 antibody
      • Two-pore channel 2 antibody
      • Voltage dependent calcium channel protein TPC2 antibody
      • Voltage-dependent calcium channel protein TPC2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPC2 antibody (ab111145)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPC2 antibody (ab111145)

      ab111145, at 3.75 µg/ml, staining TPC2 in formalin fixed, paraffin embedded Human colon tissue by Immunohistochemistry. Followed by biotinylated secondary antibody, alkaline phosphatase streptavidin and chromogen.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPC2 antibody (ab111145)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPC2 antibody (ab111145)

      ab111145, at 3.75 µg/ml, staining TPC2 in formalin fixed, paraffin embedded Human pancreas tissue by Immunohistochemistry. Followed by biotinylated secondary antibody, alkaline phosphatase streptavidin and chromogen.

    References

    ab111145 has not yet been referenced specifically in any publications.

    Publishing research using ab111145? Please let us know so that we can cite the reference in this datasheet.

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