Key features and details
- Rabbit polyclonal to TPC2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TPC2 antibody
See all TPC2 primary antibodies
DescriptionRabbit polyclonal to TPC2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human TPC2 (N terminal).
- MCF7 cell lysate, Human fetal small intestine tissue.
Protein previously labeled as Two pore calcium channel protein 2.
FormLyophilized:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituent: 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab119915 is purified by a peptide affinity column.
Our Abpromise guarantee covers the use of ab119915 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 98 kDa (predicted molecular weight: 85 kDa).|
|IHC-P||1/100 - 1/500.|
FunctionNicotinic acid adenine dinucleotide phosphate (NAADP) receptor that may function as one of the major voltage-gated Ca(2+) channels (VDCC) across the lysosomal membrane. May be involved in smooth muscle contraction.
Tissue specificityWidely expressed. Expressed at high level in liver and kidney.
Sequence similaritiesBelongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. Two pore calcium channel subfamily.
DomainEach of the two internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
Cellular localizationLysosome membrane. Only the acidic lysosomal fraction is sensitive to NAADP.
- Information by UniProt
- SHEP10 antibody
- TPC2 antibody
- TPC2_HUMAN antibody
Anti-TPC2 antibody (ab119915) at 1/500 dilution + MCF7 cell lysate
Predicted band size: 85 kDa
The amount of protein per lane: 30ug
Blocking solution: 5% (W/V) skim milk, with 1 X TBST bufferShake membrane at room temperature with ab119915, 1/500, for 30 minutes, then transfer to 4°C overnight.
ab119915 at 1/100 dilution staining TPC2 in Formalin-fixed, Paraffin-embedded Human fetal small intestine tissue by Immunohistochemistry.
ab119915 has been referenced in 1 publication.
- Lin JR et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. Elife 7:N/A (2018). PubMed: 29993362