Overview

  • Product name

  • Description

    Rabbit polyclonal to TPC2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human TPC2 (N terminal).

  • Positive control

    • MCF7 cell lysate, Human fetal small intestine tissue.
  • General notes

    Protein previously labeled as Two pore calcium channel protein 2.

Properties

  • Form

    Lyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 99% PBS, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab119915 is purified by a peptide affinity column.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab119915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 98 kDa (predicted molecular weight: 85 kDa).
IHC-P 1/100 - 1/500.

Target

  • Function

    Nicotinic acid adenine dinucleotide phosphate (NAADP) receptor that may function as one of the major voltage-gated Ca(2+) channels (VDCC) across the lysosomal membrane. May be involved in smooth muscle contraction.
  • Tissue specificity

    Widely expressed. Expressed at high level in liver and kidney.
  • Sequence similarities

    Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. Two pore calcium channel subfamily.
  • Domain

    Each of the two internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Lysosome membrane. Only the acidic lysosomal fraction is sensitive to NAADP.
  • Information by UniProt
  • Database links

  • Alternative names

    • SHEP10 antibody
    • TPC2 antibody
    • TPC2_HUMAN antibody
    • tpcn2 antibody
    • Two pore calcium channel protein 2 antibody
    • two pore segment channel 2 antibody
    • Two-pore channel 2 antibody
    • Voltage dependent calcium channel protein TPC2 antibody
    • Voltage-dependent calcium channel protein TPC2 antibody
    see all

Images

  • Anti-TPC2 antibody (ab119915) at 1/500 dilution + MCF7 cell lysate

    Predicted band size: 85 kDa



    The amount of protein per lane: 30ug

    PVDF membrane

    Blocking solution: 5% (W/V) skim milk, with 1 X TBST buffer

    Shake membrane at room temperature with ab119915, 1/500, for 30 minutes, then transfer to 4°C overnight.
  • ab119915 at 1/100 dilution staining TPC2 in Formalin-fixed, Paraffin-embedded Human fetal small intestine tissue by Immunohistochemistry.

References

This product has been referenced in:

  • Lin JR  et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. Elife 7:N/A (2018). Read more (PubMed: 29993362) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued an alternative free of charge replacement of ab81102 with the order number 1214965.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Question
Answer

Thank you for taking the time to provide this information. The details you have kindly provided will provide us with vital information for our monitoring of product quality

I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. Reviewing the details, I am sorry there are no further tips to provide on this occasion to help improve the results. I can suggest you have unfortunately received a bad vial on this occasion.

I apologize for the inconvenience and appreciate the time you have spent on these experiments. I would like to offer a refund or credit note in compensation (providing the product has been purchased within the guarantee period of 6 months). In order to arrange this, I would appreciate if you could confirm your order number and date of purchase?

Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

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Answer

Thank you for your reply and for kindly providing the image again which I have been able to access.

I see there is one clear single band in the sample lane on the blot. Because of the image quality, and because some of the bands are very faint, I am not completely sure which band in the molecular weight markers relates to which size. What size would you say the sample band is at? Because some of the marker bands are extremely faint, I am not sure if perhaps some of the smaller markers bands have run off the gel and therefore the sample band may be at the correct size?

Thank you for your cooperation. I would appreciate if you could provide further information.

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Answer

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for WB and human samples. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I am sorry I am not able to access the image you have kindly attached and would appreciate if you are able to send this again in PDF or JPEG format. This will help me to better understand the problem in this case.

Thank you for your time and cooperation. I look forward to receiving the attachment again.

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