Key features and details
- Rabbit polyclonal to TPD52
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TPD52 antibody
See all TPD52 primary antibodies
DescriptionRabbit polyclonal to TPD52
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
- ICC/IF: A431 cells. WB: RT4 and U-251 MG cell lysate. Human liver and tonsil tissue lysate. IHC-P: Human prostate, cerebral cortex and kidney tissue.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab244523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Tissue specificityIsoform 2 is expressed in colon, breast, prostate, pancreas and kidney tumor cell lines. Isoform 2 is expressed at high levels in kidney, prostate, brain, small intestine and pancreas, at moderate levels in placenta and colon, at low levels in lung, liver and heart, and at very low levels in spleen, thymus, peripheral mononuclear blood cells, testis and ovary.
Sequence similaritiesBelongs to the TPD52 family.
Developmental stageIsoform 2 is expressed at lower levels in fetal brain and kidney than in adult brain and kidney.
- Information by UniProt
- D52 antibody
- hD52 antibody
- Human 19.8 kDa protein mRNA, complete cds antibody
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for TPD52 (green) using ab244523 at 4 µg/ml in ICC/IF.
All lanes : Anti-TPD52 antibody (ab244523) at 0.4 µg/ml
Lane 1 : RT4 (Human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (Human brain glioma cell line) cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Paraffin-embedded human prostate tissue stained for TPD52 using ab244523 at 1/50 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for TPD52 using ab244523 at 1/50 dilution in immunohistochemical analysis.
Paraffin-embedded human cerebral cortex tissue stained for TPD52 using ab244523 at 1/50 dilution in immunohistochemical analysis.
Paraffin-embedded human skeletal muscle tissue stained for TPD52 using ab244523 at 1/50 dilution in immunohistochemical analysis.
Low expression as expected.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab244523 has not yet been referenced specifically in any publications.