Product nameAnti-TPD52 antibody
See all TPD52 primary antibodies
DescriptionGoat polyclonal to TPD52
Specificityab77327 is expected to recognise all three reported isoforms (NP_001020423.1, NP_001020424.1and NP_005070.1).
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Dog
- Human lymph node and human kidney lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab77327 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
Our Abpromise guarantee covers the use of ab77327 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 24 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Tissue specificityIsoform 2 is expressed in colon, breast, prostate, pancreas and kidney tumor cell lines. Isoform 2 is expressed at high levels in kidney, prostate, brain, small intestine and pancreas, at moderate levels in placenta and colon, at low levels in lung, liver and heart, and at very low levels in spleen, thymus, peripheral mononuclear blood cells, testis and ovary.
Sequence similaritiesBelongs to the TPD52 family.
Developmental stageIsoform 2 is expressed at lower levels in fetal brain and kidney than in adult brain and kidney.
- Information by UniProt
- D52 antibody
- hD52 antibody
- Human 19.8 kDa protein mRNA, complete cds antibody
Anti-TPD52 antibody (ab77327) at 1 µg/ml + human lymph node lysate in RIPA buffer at 35 µg
Predicted band size: 24 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
ab77327 has not yet been referenced specifically in any publications.