Overview

  • Product name

    Anti-TPD52 antibody [EPR14219]
    See all TPD52 primary antibodies
  • Description

    Rabbit monoclonal [EPR14219] to TPD52
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human TPD52 aa 1-100. The exact sequence is proprietary.
    Database link: P55327

  • Positive control

    • MCF7, THP1 and Raji cell lysates; Human kidney and liver tissues; MCF7 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR14219
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 22, 26 kDa (predicted molecular weight: 24 kDa).

Based on the immunogen sequence analysis, ab181260 recognizes 4 isoforms with the predicted MW of 24KDa, 20KDa, 26KDa and 22KDa, respectively.

ICC/IF 1/500.
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100.

Target

  • Tissue specificity

    Isoform 2 is expressed in colon, breast, prostate, pancreas and kidney tumor cell lines. Isoform 2 is expressed at high levels in kidney, prostate, brain, small intestine and pancreas, at moderate levels in placenta and colon, at low levels in lung, liver and heart, and at very low levels in spleen, thymus, peripheral mononuclear blood cells, testis and ovary.
  • Sequence similarities

    Belongs to the TPD52 family.
  • Developmental stage

    Isoform 2 is expressed at lower levels in fetal brain and kidney than in adult brain and kidney.
  • Information by UniProt
  • Database links

  • Alternative names

    • D52 antibody
    • hD52 antibody
    • Human 19.8 kDa protein mRNA, complete cds antibody
    • N8L antibody
    • PC 1 antibody
    • PC1 antibody
    • PC1/MRPS28 fusion protein antibody
    • PrLZ antibody
    • Prostate and colon associated protein antibody
    • Prostate leucine zipper antibody
    • Protein N8 antibody
    • TPD52 antibody
    • TPD52_HUMAN antibody
    • Tumor protein D52 antibody
    see all

Images

  • All lanes : Anti-TPD52 antibody [EPR14219] (ab181260) at 1/2000 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : THP1 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 24 kDa

  • Immunocytochemistry/Immunofluorescence analysis of THP-1 cells labelling TPD52 with ab181260 at 1/1000. Cells were fixed with 100% Paraformaldehyde. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.

    Control: PBS only.
    Nuclear counter stain: DAPI.

  • Anti-TPD52 antibody [EPR14219] (ab181260) at 1/5000 dilution + Raji cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 24 kDa

  • Immunohistochemical analysis of paraffin-embedded Human Kidney tissue labeling TPD52 with ab181260 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Human Liver tissue labeling TPD52 with ab181260 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

  • Flow cytometric analysis of MCF7 cells (2% paraformaldehyde-fixed) labeling TPD52 with ab181260 at 1/10 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

References

This product has been referenced in:

  • Zhang Y  et al. Long non-coding RNA ferritin heavy polypeptide 1 pseudogene 3 controls glioma cell proliferation and apoptosis via regulation of the microRNA-224-5p/tumor protein D52 axis. Mol Med Rep 18:4239-4246 (2018). Read more (PubMed: 30221720) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181260.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up