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RecombinantRabMAb

Recombinant Anti-TPD52 antibody [EPR14220] (ab182578)

Submit a review Submit a question References (7)
Western blot - Anti-TPD52 antibody [EPR14220] (ab182578)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52 antibody [EPR14220] (ab182578)
  • Immunocytochemistry/ Immunofluorescence - Anti-TPD52 antibody [EPR14220] (ab182578)
  • Flow Cytometry (Intracellular) - Anti-TPD52 antibody [EPR14220] (ab182578)
  • Immunoprecipitation - Anti-TPD52 antibody [EPR14220] (ab182578)
  • Western blot - Anti-TPD52 antibody [EPR14220] (ab182578)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52 antibody [EPR14220] (ab182578)
  • Anti-TPD52 antibody [EPR14220] (ab182578)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14220] to TPD52
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP, IHC-P
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

APC Carrier Free HRP PE

Overview

  • Product name

    Anti-TPD52 antibody [EPR14220]
    See all TPD52 primary antibodies

  • Description

    Rabbit monoclonal [EPR14220] to TPD52

  • Host species

    Rabbit

  • Specificity

    Based on immunogen sequence analysis, ab182578 recognizes 4 isoforms with the predicted MWs of 24KDa, 20KDa, 26 KDa and 22KDa, respectively.

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: SW480, HCT-116, Raji and THP1 cell lysates. IHC-P: Human prostatic hyperplasia and mouse kidney tissues. ICC/IF: MCF7 cells. Flow Cyt (intra): Raji cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14220

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab182578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/350.
WB
1/10000 - 1/50000. Detects a band of approximately 20-26 kDa (predicted molecular weight: 24 kDa).
ICC/IF
1/500.
IP
1/40 - 1/60.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
Flow Cyt (Intra)
1/350.
WB
1/10000 - 1/50000. Detects a band of approximately 20-26 kDa (predicted molecular weight: 24 kDa).
ICC/IF
1/500.
IP
1/40 - 1/60.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Tissue specificity

    Isoform 2 is expressed in colon, breast, prostate, pancreas and kidney tumor cell lines. Isoform 2 is expressed at high levels in kidney, prostate, brain, small intestine and pancreas, at moderate levels in placenta and colon, at low levels in lung, liver and heart, and at very low levels in spleen, thymus, peripheral mononuclear blood cells, testis and ovary.

  • Sequence similarities

    Belongs to the TPD52 family.

  • Developmental stage

    Isoform 2 is expressed at lower levels in fetal brain and kidney than in adult brain and kidney.
  • Information by UniProt

  • Database links

    • Alternative names

      • D52 antibody
      • hD52 antibody
      • Human 19.8 kDa protein mRNA, complete cds antibody
      • N8L antibody
      • PC 1 antibody
      • PC1 antibody
      • PC1/MRPS28 fusion protein antibody
      • PrLZ antibody
      • Prostate and colon associated protein antibody
      • Prostate leucine zipper antibody
      • Protein N8 antibody
      • TPD52 antibody
      • TPD52_HUMAN antibody
      • Tumor protein D52 antibody
      see all

    Images

    • Western blot - Anti-TPD52 antibody [EPR14220] (ab182578)
      Western blot - Anti-TPD52 antibody [EPR14220] (ab182578)
      All lanes : Anti-TPD52 antibody [EPR14220] (ab182578) at 1/20000 dilution

      Lane 1 : HCT-116 cell lysate
      Lane 2 : Raji cell lysate
      Lane 3 : THP1 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 24 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52 antibody [EPR14220] (ab182578)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52 antibody [EPR14220] (ab182578)

      Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling TPD52 using ab182578 at 1/100 dilution. A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-TPD52 antibody [EPR14220] (ab182578)
      Immunocytochemistry/ Immunofluorescence - Anti-TPD52 antibody [EPR14220] (ab182578)

      Immunofluorescent analysis of MCF7 cells labeling TPD52 using ab182578 at 1/500 dilution. A Goat anti rabbit IgG (Alexa Fluor555) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde. Counterstain: DAPI.

    • Flow Cytometry (Intracellular) - Anti-TPD52 antibody [EPR14220] (ab182578)
      Flow Cytometry (Intracellular) - Anti-TPD52 antibody [EPR14220] (ab182578)

      Intracellular Flow Cytometry analysis of Raji (human Burkitt's lymphoma) cells labeling TPD52 (red) with ab182578 at a 1/350 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies. 

       

       

    • Immunoprecipitation - Anti-TPD52 antibody [EPR14220] (ab182578)
      Immunoprecipitation - Anti-TPD52 antibody [EPR14220] (ab182578)

      Western blot analysis of immunoprecipitation pellet from HCT-116 cell lysate (+) or a negative control (-) immunoprecipitated using ab182578 at 1/50 dilution.
      Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

    • Western blot - Anti-TPD52 antibody [EPR14220] (ab182578)
      Western blot - Anti-TPD52 antibody [EPR14220] (ab182578)
      Anti-TPD52 antibody [EPR14220] (ab182578) at 1/10000 dilution + SW480 cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 24 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52 antibody [EPR14220] (ab182578)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52 antibody [EPR14220] (ab182578)

      Immunohistochemical analysis of paraffin-embedded Human Prostatic hyperplasia tissue labeling TPD52 using ab182578 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Anti-TPD52 antibody [EPR14220] (ab182578)
      Anti-TPD52 antibody [EPR14220] (ab182578)

    References (7)

    Publishing research using ab182578? Please let us know so that we can cite the reference in this datasheet.

    ab182578 has been referenced in 7 publications.

    • Abe Y  et al. Tumor protein D52 is upregulated in oral squamous carcinoma cells under hypoxia in a hypoxia-inducible-factor-independent manner and is involved in cell death resistance. Cell Biosci 11:122 (2021). PubMed: 34217360
    • Shi P  et al. Hsa_circ_0084927 Regulates Cervical Cancer Advancement via Regulation of the miR-634/TPD52 Axis. Cancer Manag Res 12:9435-9448 (2020). PubMed: 33061631
    • Liu S & Xi X LINC01133 contribute to epithelial ovarian cancer metastasis by regulating miR-495-3p/TPD52 axis. Biochem Biophys Res Commun 533:1088-1094 (2020). PubMed: 33036757
    • Fu M  et al. MicroRNA-103a-3p promotes metastasis by targeting TPD52 in salivary adenoid cystic carcinoma. Int J Oncol 57:574-586 (2020). PubMed: 32467999
    • Bai Y  et al. Lipid storage and lipophagy regulates ferroptosis. Biochem Biophys Res Commun 508:997-1003 (2019). PubMed: 30545638
    • Wang G  et al. miR-107 Enhances the Sensitivity of Breast Cancer Cells to Paclitaxel. Open Med (Wars) 14:456-466 (2019). PubMed: 31206033
    • Kato K  et al. Opposite effects of tumor protein D (TPD) 52 and TPD54 on oral squamous cell carcinoma cells. Int J Oncol 50:1634-1646 (2017). PubMed: 28339026

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