The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Detects a band of approximately 20-26 kDa (predicted molecular weight: 24 kDa).
1/40 - 1/60.
1/50 - 1/100.
Isoform 2 is expressed in colon, breast, prostate, pancreas and kidney tumor cell lines. Isoform 2 is expressed at high levels in kidney, prostate, brain, small intestine and pancreas, at moderate levels in placenta and colon, at low levels in lung, liver and heart, and at very low levels in spleen, thymus, peripheral mononuclear blood cells, testis and ovary.
Belongs to the TPD52 family.
Isoform 2 is expressed at lower levels in fetal brain and kidney than in adult brain and kidney.
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling TPD52 using ab182578 at 1/100 dilution. A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin.
Immunofluorescent analysis of MCF7 cells labeling TPD52 using ab182578 at 1/500 dilution. A Goat anti rabbit IgG (Alexa Fluor555) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde. Counterstain: DAPI.
Flow cytometry analysis of Raji (human Burkitt's lymphoma) cells labeling TPD52 (red) with ab182578 at a 1/350 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.
Western blot analysis of immunoprecipitation pellet from HCT-116 cell lysate (+) or a negative control (-) immunoprecipitated using ab182578 at 1/50 dilution. Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Western blot - Anti-TPD52 antibody [EPR14220] (ab182578)
Anti-TPD52 antibody [EPR14220] (ab182578) at 1/10000 dilution + SW480 cell lysate at 20 µg
Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human Prostatic hyperplasia tissue labeling TPD52 using ab182578 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.