Recombinant Anti-TPD52 antibody [EPR14220] - BSA and Azide free (ab250629)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14220] to TPD52 - BSA and Azide free
- Suitable for: ICC/IF, IHC-P, Flow Cyt (Intra), IP, WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-TPD52 antibody [EPR14220] - BSA and Azide free
See all TPD52 primary antibodies -
Description
Rabbit monoclonal [EPR14220] to TPD52 - BSA and Azide free -
Host species
Rabbit -
Specificity
Based on immunogen sequence analysis, this antibody recognizes 4 isoforms with the predicted MWs of 24KDa, 20KDa, 26 KDa and 22KDa, respectively.
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Tested applications
Suitable for: ICC/IF, IHC-P, Flow Cyt (Intra), IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250629 is the carrier-free version of ab182578.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14220 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-TPD52 antibody [EPR14220] (ab182578)
- Alexa Fluor® 488 Anti-TPD52 antibody [EPR14220] (ab309734)
- Alexa Fluor® 647 Anti-TPD52 antibody [EPR14220] (ab310100)
- Alexa Fluor® 594 Anti-TPD52 antibody [EPR14220] (ab310505)
- Alexa Fluor® 555 Anti-TPD52 antibody [EPR14220] (ab312034)
- Alexa Fluor® 568 Anti-TPD52 antibody [EPR14220] (ab312511)
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250629 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 20-26 kDa (predicted molecular weight: 24 kDa).
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 20-26 kDa (predicted molecular weight: 24 kDa). |
Target
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Tissue specificity
Isoform 2 is expressed in colon, breast, prostate, pancreas and kidney tumor cell lines. Isoform 2 is expressed at high levels in kidney, prostate, brain, small intestine and pancreas, at moderate levels in placenta and colon, at low levels in lung, liver and heart, and at very low levels in spleen, thymus, peripheral mononuclear blood cells, testis and ovary. -
Sequence similarities
Belongs to the TPD52 family. -
Developmental stage
Isoform 2 is expressed at lower levels in fetal brain and kidney than in adult brain and kidney. - Information by UniProt
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Database links
- Entrez Gene: 7163 Human
- Entrez Gene: 21985 Mouse
- Entrez Gene: 294900 Rat
- Omim: 604068 Human
- SwissProt: P55327 Human
- SwissProt: Q62393 Mouse
- Unigene: 368433 Human
- Unigene: 371590 Mouse
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Alternative names
- D52 antibody
- hD52 antibody
- Human 19.8 kDa protein mRNA, complete cds antibody
see all
Images
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All lanes : Anti-TPD52 antibody [EPR14220] (ab182578) at 1/20000 dilution
Lane 1 : HCT-116 cell lysate
Lane 2 : Raji cell lysate
Lane 3 : THP1 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 24 kDaThis data was developed using ab182578, the same antibody clone in a different buffer formulation.
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This data was developed using ab182578, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling TPD52 using ab182578 at 1/100 dilution. A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol. -
This data was developed using ab182578, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of MCF7 cells labeling TPD52 using ab182578 at 1/500 dilution. A Goat anti rabbit IgG (Alexa Fluor555) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde. Counterstain: DAPI.
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This data was developed using ab182578, the same antibody clone in a different buffer formulation.
Intracellular Flow Cytometry analysis of Raji (human Burkitt's lymphoma) cells labeling TPD52 (red) with ab182578 at a 1/350 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.
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This data was developed using ab182578, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from HCT-116 cell lysate (+) or a negative control (-) immunoprecipitated using ab182578 at 1/50 dilution.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution. -
This data was developed using ab182578, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human Prostatic hyperplasia tissue labeling TPD52 using ab182578 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250629 has not yet been referenced specifically in any publications.