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  1. Link

    tpd52l2-antibody-ab234819.pdf

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Anti-TPD52L2 antibody (ab234819)

  • Datasheet
  • SDS
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Abpromise

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Western blot - Anti-TPD52L2 antibody (ab234819)
  • Immunocytochemistry/ Immunofluorescence - Anti-TPD52L2 antibody (ab234819)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52L2 antibody (ab234819)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52L2 antibody (ab234819)

Key features and details

  • Rabbit polyclonal to TPD52L2
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-TPD52L2 antibody
    See all TPD52L2 primary antibodies
  • Description

    Rabbit polyclonal to TPD52L2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Orangutan
  • Immunogen

    Recombinant full length protein corresponding to Human TPD52L2 aa 1-206.
    Sequence:

    MDSAGQDINLNSPNKGLLSDSMTDVPVDTGVAARTPAVEGLTEAEEEELR AELTKVEEEIVTLRQVLAAKERHCGELKRRLGLSTLGELKQNLSRSWHDV QVSSAYVKTSEKLGEWNEKVTQSDLYKKTQETLSQAGQKTSAALSTVGSA ISRKLGDMRNSATFKSFEDRVGTIKSKVVGDRENGSDNLPSSAGSGDKPL SDPAPF


    Database link: O43399
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human pancreatic cancer and spleen tissues. ICC/IF: HepG2 cells. WB: Mouse spleen lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell differentiation
    • Cancer
    • Cell cycle
    • Cell division
    • Cancer
    • Tumor biomarkers
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Mouse spleen tissue lysate - total protein (ab29293)
    • Mouse spleen tissue lysate - total protein (ab7937)

Applications

Our Abpromise guarantee covers the use of ab234819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/5000. Predicted molecular weight: 22 kDa.
IHC-P 1/20 - 1/200.
ICC/IF 1/50 - 1/200.

Target

  • Sequence similarities

    Belongs to the TPD52 family.
  • Target information above from: UniProt accession O43399 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7165 Human
    • Entrez Gene: 66314 Mouse
    • Entrez Gene: 100172104 Orangutan
    • Omim: 603747 Human
    • SwissProt: O43399 Human
    • SwissProt: Q9CYZ2 Mouse
    • SwissProt: Q5RCT1 Orangutan
    • Unigene: 473296 Human
    • Unigene: 136648 Mouse
    see all
  • Alternative names

    • 2810411G23Rik antibody
    • AU016537 antibody
    • C86069 antibody
    • D54 antibody
    • DKFZp686A1765 antibody
    • HCCR binding protein 2 antibody
    • hD54 antibody
    • MGC73020 antibody
    • RP23 33L3.2 antibody
    • TPD52L2 antibody
    • TPD54 antibody
    • TPD54_HUMAN antibody
    • tumor protein D52 like 2 antibody
    • Tumor protein D52-like 2 antibody
    • Tumor protein D54 antibody
    see all

Images

  • Western blot - Anti-TPD52L2 antibody (ab234819)
    Western blot - Anti-TPD52L2 antibody (ab234819)
    Anti-TPD52L2 antibody (ab234819) at 1/200 dilution + Mouse spleen lysate

    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 22 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-TPD52L2 antibody (ab234819)
    Immunocytochemistry/ Immunofluorescence - Anti-TPD52L2 antibody (ab234819)

    HepG2 (human liver hepatocellular carcinoma cell line) cells stained for TPD52L2 green) using ab234819 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52L2 antibody (ab234819)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52L2 antibody (ab234819)

    Paraffin-embedded human pancreatic cancer tissue stained for TPD52L2 using ab234819 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52L2 antibody (ab234819)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPD52L2 antibody (ab234819)

    Paraffin-embedded human spleen tissue stained for TPD52L2 using ab234819 at 1/100 dilution in immunohistochemical analysis.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab234819? Please let us know so that we can cite the reference in this datasheet.

    ab234819 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews

    Western blot abreview for Anti-TPD52L2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    HeLa
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 09 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TPD52L2 antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.2% Saponin
    Specification
    HeLa
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted May 28 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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