Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.2% Saponin
Specification
HeLa
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: Blocking
Dilution
1/100
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: anti Rb from invitrogen
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
localizes to punch and cytoplasm? There is quite a bot of background
Abcam response
Thank you for submitting your feedback using our Abreview system. To improve your results, you might try a different method of permeabilization and decreasing the incubation time of the primary to 30 minutes. This antibody is guarantee for use In ICC/IF and human samples, and we are happy to provide a replacement or refund if it is not working as it should!
Abcam user community
Verified customer
Submitted May 28 2019