Recombinant Anti-TPGS1 antibody [EPR14324] (ab184178)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14324] to TPGS1
- Suitable for: Flow Cyt (Intra), WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-TPGS1 antibody [EPR14324]
See all TPGS1 primary antibodies -
Description
Rabbit monoclonal [EPR14324] to TPGS1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Rat testis, Mouse testis or SH-SY5Y cell lysates, SH-SY5Y cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR14324 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184178 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/50.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
|
|
IP |
1/20.
|
Notes |
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Flow Cyt (Intra)
1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa). |
IP
1/20. |
Target
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Relevance
TPGS1 may act in the targeting of the tubulin polyglutamylase complex. It is required for the development of the spermatid flagellum. -
Cellular localization
Microtubule. -
Database links
- Entrez Gene: 91978 Human
- Entrez Gene: 110012 Mouse
- Entrez Gene: 691093 Rat
- SwissProt: Q6ZTW0 Human
- SwissProt: Q99MS8 Mouse
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Alternative names
- C19orf20 antibody
- Gene trap ROSA b geo 22 antibody
- GTRGEO22 antibody
see all
Images
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Anti-TPGS1 antibody [EPR14324] (ab184178) at 1/10000 dilution + Mouse testis tissue lysate at 20 µg
Secondary
Peroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 31 kDa -
All lanes : Anti-TPGS1 antibody [EPR14324] (ab184178) at 1/2000 dilution
Lane 1 : Rat testis tissue lysate
Lane 2 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 31 kDa -
Immunoprecipitation analysis of SH-SY5Y cells labeling TPGS1 using ab184178 at 1/50 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 was used as secondary antibody.
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Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixed SH-SY5Y cells staining TPGS1 using ab184178 at 1/50 and FITC conjugated Goat anti rabbit IgG at 1/150 as a secondary antibody (red curve), and a Rabbit monoclonal IgG negative control (green curve).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab184178 has been referenced in 1 publication.
- Huttlin EL et al. Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 184:3022-3040.e28 (2021). PubMed: 33961781