Overview

  • Product name

    Anti-TPGS1 antibody [EPR14324]
    See all TPGS1 primary antibodies
  • Description

    Rabbit monoclonal [EPR14324] to TPGS1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human TPGS1 aa 1-150. The exact sequence is proprietary.
    Database link: Q6ZTW0

  • Positive control

    • Rat testis, Mouse testis or SH-SY5Y cell lysates, SH-SY5Y cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR14324
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab184178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
IP 1/20.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance

    TPGS1 may act in the targeting of the tubulin polyglutamylase complex. It is required for the development of the spermatid flagellum.
  • Cellular localization

    Microtubule.
  • Database links

  • Alternative names

    • C19orf20 antibody
    • Gene trap ROSA b geo 22 antibody
    • GTRGEO22 antibody
    • PGs1 antibody
    • Tubulin polyglutamylase complex subunit 1 antibody
    see all

Images

  • Anti-TPGS1 antibody [EPR14324] (ab184178) at 1/10000 dilution + Mouse testis lysate at 20 µg

    Secondary
    Peroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 31 kDa

  • All lanes : Anti-TPGS1 antibody [EPR14324] (ab184178) at 1/2000 dilution

    Lane 1 : Rat testis lysate
    Lane 2 : SH-SY5Y lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 31 kDa

  • Immunoprecipitation analysis of SH-SY5Y cells labeling TPGS1 using ab184178 at 1/50 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 was used as secondary antibody.

  • Flow cytometry analysis of 2% paraformaldehyde fixed SH-SY5Y cells staining TPGS1 using ab184178 at 1/50 and FITC conjugated Goat anti rabbit IgG at 1/150 as a secondary antibody (red curve), and a Rabbit monoclonal IgG negative control (green curve).

References

ab184178 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab184178.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up