Anti-TPH2 antibody (ab121013)
Key features and details
- Goat polyclonal to TPH2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-TPH2 antibody
See all TPH2 primary antibodies -
Description
Goat polyclonal to TPH2 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Rabbit, Horse, Hamster, Cow, Dog, Pig, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human TPH2 aa 1-100 (N terminal) (Cysteine residue). NP_775489.2
Database link: Q8IWU9 -
Positive control
- WB: HEK293 lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab121013 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121013 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Tissue specificity
Brain specific. -
Pathway
Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2. -
Involvement in disease
Genetic variation in TPH2 may influence susceptibility to major depressive disorder (MDD) [MIM:608516].
Defects in TPH2 are the cause of susceptibility to attention deficit-hyperactivity disorder type 7 (ADHD7) [MIM:613003]. ADHD is a neurobehavioral developmental disorder and is primarily characterized by the co-existence of attentional problems and hyperactivity, with each behavior occurring infrequently alone. Note=Naturally occurring variants of TPH2 with impaired enzyme activity could cause deficiency of serotonin production and result in an increased risk of developing behavioral disorders. -
Sequence similarities
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
Contains 1 ACT domain. - Information by UniProt
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Database links
- Entrez Gene: 618062 Cow
- Entrez Gene: 121278 Human
- Entrez Gene: 317675 Rat
- Omim: 607478 Human
- SwissProt: Q2KIQ5 Cow
- SwissProt: Q8IWU9 Human
- SwissProt: Q8CGU9 Rat
- Unigene: 376337 Human
see all -
Alternative names
- MGC138871 antibody
- ADHD7 antibody
- FLJ37295 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab121013 has been referenced in 10 publications.
- Takahashi A et al. Lateral habenula glutamatergic neurons projecting to the dorsal raphe nucleus promote aggressive arousal in mice. Nat Commun 13:4039 (2022). PubMed: 35864121
- Leitner DF et al. Raphe and ventrolateral medulla proteomics in epilepsy and sudden unexpected death in epilepsy. Brain Commun 4:fcac186 (2022). PubMed: 35928051
- Patodia S et al. Medullary tyrosine hydroxylase catecholaminergic neuronal populations in sudden unexpected death in epilepsy. Brain Pathol 31:133-143 (2021). PubMed: 32852867
- Li L et al. Dorsal raphe nucleus to anterior cingulate cortex 5-HTergic neural circuit modulates consolation and sociability. Elife 10:N/A (2021). PubMed: 34080539
- Burg T et al. Absence of Subcerebral Projection Neurons Is Beneficial in a Mouse Model of Amyotrophic Lateral Sclerosis. Ann Neurol 88:688-702 (2020). PubMed: 32588450
- Farrelly LA et al. Histone serotonylation is a permissive modification that enhances TFIID binding to H3K4me3. Nature 567:535-539 (2019). PubMed: 30867594
- Jiao H et al. Influence of Xiaoyaosan on depressive-like behaviors in chronic stress-depressed rats through regulating tryptophan metabolism in hippocampus. Neuropsychiatr Dis Treat 15:21-31 (2019). PubMed: 30587994
- Patodia S et al. The ventrolateral medulla and medullary raphe in sudden unexpected death in epilepsy. Brain 141:1719-1733 (2018). PubMed: 29608654
- Li Z et al. Generation of tryptophan hydroxylase 2 gene knockout pigs by CRISPR/Cas9-mediated gene targeting. J Biomed Res 31:445-452 (2017). PubMed: 28866660
- El Oussini H et al. Degeneration of serotonin neurons triggers spasticity in amyotrophic lateral sclerosis. Ann Neurol 82:444-456 (2017). PubMed: 28856708