Key features and details
- Goat polyclonal to TPH2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-TPH2 antibody
See all TPH2 primary antibodies
DescriptionGoat polyclonal to TPH2
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Horse, Hamster, Cow, Dog, Pig, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
- WB: HEK293 lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab121013 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab121013 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/800 - 1/1500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa).
1 hour primary incubation is recommended for this product.
Tissue specificityBrain specific.
PathwayAromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.
Involvement in diseaseGenetic variation in TPH2 may influence susceptibility to major depressive disorder (MDD) [MIM:608516].
Defects in TPH2 are the cause of susceptibility to attention deficit-hyperactivity disorder type 7 (ADHD7) [MIM:613003]. ADHD is a neurobehavioral developmental disorder and is primarily characterized by the co-existence of attentional problems and hyperactivity, with each behavior occurring infrequently alone. Note=Naturally occurring variants of TPH2 with impaired enzyme activity could cause deficiency of serotonin production and result in an increased risk of developing behavioral disorders.
Sequence similaritiesBelongs to the biopterin-dependent aromatic amino acid hydroxylase family.
Contains 1 ACT domain.
- Information by UniProt
- MGC138871 antibody
- ADHD7 antibody
- FLJ37295 antibody
IHC-P image of THP2 staining on mouse sections of Substantia Nigra. The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were them blocked using 1% BSA for 10 mins at 21°C. ab121013 was diluted 1:800and incubated with the sections for 2 hours at 21°C. The secondary antibody used was rabbit polyclonal to goat IgG conjugated to HRP.
IHC-P image of THP2 staining on rat sections of nigral-striatal track . The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were them blocked using 1% BSA for 10 mins at 21°C. ab121013 was diluted 1:1500 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was rabbit polyclonal to goat IgG conjugated to HRP.
Anti-TPH2 antibody (ab121013) at 0.5 µg/ml + HEK293 lysate in RIPA buffer at 35 µg
Predicted band size: 56 kDa
ab121013 has been referenced in 5 publications.
- Jiao H et al. Influence of Xiaoyaosan on depressive-like behaviors in chronic stress-depressed rats through regulating tryptophan metabolism in hippocampus. Neuropsychiatr Dis Treat 15:21-31 (2019). PubMed: 30587994
- Farrelly LA et al. Histone serotonylation is a permissive modification that enhances TFIID binding to H3K4me3. Nature 567:535-539 (2019). PubMed: 30867594
- Patodia S et al. The ventrolateral medulla and medullary raphe in sudden unexpected death in epilepsy. Brain 141:1719-1733 (2018). PubMed: 29608654
- Li Z et al. Generation of tryptophan hydroxylase 2 gene knockout pigs by CRISPR/Cas9-mediated gene targeting. J Biomed Res 31:445-452 (2017). PubMed: 28866660
- El Oussini H et al. Degeneration of serotonin neurons triggers spasticity in amyotrophic lateral sclerosis. Ann Neurol 82:444-456 (2017). PubMed: 28856708