Overview

  • Product name

    Anti-TPH2 antibody [EPR19191]
    See all TPH2 primary antibodies
  • Description

    Rabbit monoclonal [EPR19191] to TPH2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IP, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Mouse TPH2 aa 1-250. The exact sequence is proprietary.
    Database link: Q8CGV2

  • Positive control

    • WB: Mouse midbrain and brainstem lysates. IHC-P: Mouse and rat brainstem tissues. IHC-Fr: Mouse brainstem tissue. IP: Mouse brain lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19191
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab184505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IHC-P 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/40.
IHC-Fr 1/500.

Target

  • Tissue specificity

    Brain specific.
  • Pathway

    Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.
  • Involvement in disease

    Genetic variation in TPH2 may influence susceptibility to major depressive disorder (MDD) [MIM:608516].
    Defects in TPH2 are the cause of susceptibility to attention deficit-hyperactivity disorder type 7 (ADHD7) [MIM:613003]. ADHD is a neurobehavioral developmental disorder and is primarily characterized by the co-existence of attentional problems and hyperactivity, with each behavior occurring infrequently alone. Note=Naturally occurring variants of TPH2 with impaired enzyme activity could cause deficiency of serotonin production and result in an increased risk of developing behavioral disorders.
  • Sequence similarities

    Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
    Contains 1 ACT domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • MGC138871 antibody
    • ADHD7 antibody
    • FLJ37295 antibody
    • MGC138872 antibody
    • Neuronal tryptophan hydroxylase antibody
    • NTPH antibody
    • TPH 2 antibody
    • Tph2 antibody
    • TPH2_HUMAN antibody
    • Tryptophan 5-hydroxylase 2 antibody
    • Tryptophan 5-monooxygenase 2 antibody
    • Tryptophan hydroxylase 2 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Mouse brainstem tissue labeling TPH2 with ab184505 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on dorsal raphe cells of mouse brainstem [PMID: 22912815]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • All lanes : Anti-TPH2 antibody [EPR19191] (ab184505) at 1/1000 dilution

    Lane 1 : Mouse midbrain lysate
    Lane 2 : Mouse brainstem lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Mouse spleen lysate
    Lane 5 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 6 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 56 kDa
    Observed band size: 56 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    This target is specifically expressed in DR (dorsal raphe nucleus). WB was tested with midbrain or brainstem because both include the DR zone. The results were consistent with the literature (PMID:21272616; PMID: 19181488).

  • Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling TPH2 with ab184505 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. No staining on mouse spleen. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Rat brainstem tissue labeling TPH2 with ab184505 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on dorsal raphe cells of rat brainstem [PMID: 22912815]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling TPH2 with ab184505 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. No staining on rat stomach. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse brainstem tissue labeling TPH2 with ab184505 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasm staining on dorsal raphe cells of mouse brainstem [PMID: 22912815]. The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 secondary antibody at 1/1000 dilution.

  • TPH2 was immunoprecipitated from 0.35 mg of Mouse brain lysate with ab184505 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184505 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Mouse brain lysate, 10µg (Input).

    Lane 2: ab184505 IP in Mouse brain lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab184505 in Mouse brain lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
brain
Blocking step
Odyssey blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

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Verified customer

Submitted Oct 04 2019

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