Anti-Tpit antibody (ab155554)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Tpit antibody
See all Tpit primary antibodies -
Description
Rabbit polyclonal to Tpit -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Chicken, Cow, Dog
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-264 of Human Tpit (Uniprot ID: O60806).
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Positive control
- 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysates.
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General notes
Keep as concentrated solution.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab155554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 48 kDa. |
Target
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Relevance
Tpit (TBX19, T box 19) is a highly cell-restricted transcription factor belonging to the T-Box family. T box 19 is required for pro-opeiomelanocortin (POMC) gene expression and differentiation of corticotroph and melanotroph cells. Tpit gene mutations have been shown to be directly correlated to pituitary adrenocorticotropin (ACTH) deficiencies. Tpit functions as a transcriptional activator by recruiting the p160 family of coactivators. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 395793 Chicken
- Entrez Gene: 514352 Cow
- Entrez Gene: 449029 Dog
- Entrez Gene: 9095 Human
- Entrez Gene: 83993 Mouse
- Omim: 604614 Human
- SwissProt: O60806 Human
- SwissProt: Q99ME7 Mouse
see all -
Alternative names
- DJ747L4.1 antibody
- T Box 19 antibody
- T box factor, pituitary antibody
see all
Images
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Western blot - Anti-Tpit antibody (ab155554)Anti-Tpit antibody (ab155554) at 1/5000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 48 kDa
10% SDS PAGE
Protocols
References
ab155554 has not yet been referenced specifically in any publications.
