Anti-Tpit antibody (ab155554)
Key features and details
- Rabbit polyclonal to Tpit
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Tpit antibody
See all Tpit primary antibodies -
Description
Rabbit polyclonal to Tpit -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Chicken, Cow, Dog
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-264 of Human Tpit (Uniprot ID: O60806).
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Positive control
- 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysates.
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General notes
Keep as concentrated solution.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab155554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 48 kDa. |
Target
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Relevance
Tpit (TBX19, T box 19) is a highly cell-restricted transcription factor belonging to the T-Box family. T box 19 is required for pro-opeiomelanocortin (POMC) gene expression and differentiation of corticotroph and melanotroph cells. Tpit gene mutations have been shown to be directly correlated to pituitary adrenocorticotropin (ACTH) deficiencies. Tpit functions as a transcriptional activator by recruiting the p160 family of coactivators. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 395793 Chicken
- Entrez Gene: 514352 Cow
- Entrez Gene: 449029 Dog
- Entrez Gene: 9095 Human
- Entrez Gene: 83993 Mouse
- Omim: 604614 Human
- SwissProt: O60806 Human
- SwissProt: Q99ME7 Mouse
see all -
Alternative names
- DJ747L4.1 antibody
- T Box 19 antibody
- T box factor, pituitary antibody
see all
Images
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Western blot - Anti-Tpit antibody (ab155554)Anti-Tpit antibody (ab155554) at 1/5000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 48 kDa
10% SDS PAGE
References (0)
ab155554 has not yet been referenced specifically in any publications.
