- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Tpit antibody
See all Tpit primary antibodies -
DescriptionRabbit polyclonal to Tpit
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Chicken, Cow, Dog
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-264 of Human Tpit (Uniprot ID: O60806).
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Positive control
- 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysates.
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General notesKeep as concentrated solution.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab155554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 48 kDa. |
Target
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RelevanceTpit (TBX19, T box 19) is a highly cell-restricted transcription factor belonging to the T-Box family. T box 19 is required for pro-opeiomelanocortin (POMC) gene expression and differentiation of corticotroph and melanotroph cells. Tpit gene mutations have been shown to be directly correlated to pituitary adrenocorticotropin (ACTH) deficiencies. Tpit functions as a transcriptional activator by recruiting the p160 family of coactivators.
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Cellular localizationNuclear
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Database links
- Entrez Gene: 395793 Chicken
- Entrez Gene: 514352 Cow
- Entrez Gene: 449029 Dog
- Entrez Gene: 9095 Human
- Entrez Gene: 83993 Mouse
- Omim: 604614 Human
- SwissProt: O60806 Human
- SwissProt: Q99ME7 Mouse
see all -
Alternative names
- DJ747L4.1 antibody
- T Box 19 antibody
- T box factor, pituitary antibody
see all
Images
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Western blot - Anti-Tpit antibody (ab155554)Anti-Tpit antibody (ab155554) at 1/5000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 48 kDa
10% SDS PAGE
Protocols
References
ab155554 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"