Key features and details
- Rabbit polyclonal to TPK1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-TPK1 antibody
See all TPK1 primary antibodies
DescriptionRabbit polyclonal to TPK1
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant full length protein corresponding to Human TPK1 aa 1-243.
MEHAFTPLEPLLSTGNLKYCLVILNQPLDNYFRHLWNKALLRACADGGAN RLYDITEGERESFLPEFINGDFDSIRPEVREYYATKGCELISTPDQDHTD FTKCLKMLQKKIEEKDLKVDVIVTLGGLAGRFDQIMASVNTLFQATHITP FPIIIIQEESLIYLLQPGKHRLHVDTGMEGDWCGLIPVGQPCMQVTTTGL KWNLTNDVLAFGTLVSTSNTYDGSGVVTVETDHPLLWTMAIKS
Database link: Q9H3S4-1
- WB: Mouse liver and kidney tissue lysate. IHC-P: Human kidney and heart tissue. ICC/IF: PC-3 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230263 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/5000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).|
|ICC/IF||1/50 - 1/200.|
FunctionCatalyzes the phosphorylation of thiamine to thiamine pyrophosphate. Can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate.
Tissue specificityDetected in heart, kidney, testis, small intestine and peripheral blood leukocytes, and at very low levels in a variety of tissues.
PathwayCofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine diphosphate from thiamine: step 1/1.
Sequence similaritiesBelongs to the thiamine pyrophosphokinase family.
- Information by UniProt
- hTPK1 antibody
- Placental protein 20 antibody
- PP20 antibody
All lanes : Anti-TPK1 antibody (ab230263) at 1/1000 dilution
Lane 1 : Mouse liver tissue lysate.
Lane 2 : Mouse kidney tissue lysate.
All lanes : Goat anti-rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 27 kDa
Observed band size: 27 kDa
PC-3 (human prostate adenocarcinoma cell line) cells stained for TPK1 (green) using ab230263 at 1/100 dilution in ICC/IF. Secondary used is an Alexa Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
Paraffin-embedded human kidney tissue stained for TPK1 using ab230263 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human heart tissue stained for TPK1 using ab230263 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230263 has not yet been referenced specifically in any publications.