Overview

  • Product name

  • Description

    Rabbit polyclonal to TPK1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein corresponding to Human TPK1 aa 1-243.
    Sequence:

    MEHAFTPLEPLLSTGNLKYCLVILNQPLDNYFRHLWNKALLRACADGGAN RLYDITEGERESFLPEFINGDFDSIRPEVREYYATKGCELISTPDQDHTD FTKCLKMLQKKIEEKDLKVDVIVTLGGLAGRFDQIMASVNTLFQATHITP FPIIIIQEESLIYLLQPGKHRLHVDTGMEGDWCGLIPVGQPCMQVTTTGL KWNLTNDVLAFGTLVSTSNTYDGSGVVTVETDHPLLWTMAIKS


    Database link: Q9H3S4-1

  • Positive control

    • WB: Mouse liver and kidney tissue lysate. IHC-P: Human kidney and heart tissue. ICC/IF: PC-3 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab230263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/200.
WB 1/500 - 1/5000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
ICC/IF 1/50 - 1/200.

Target

  • Function

    Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. Can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate.
  • Tissue specificity

    Detected in heart, kidney, testis, small intestine and peripheral blood leukocytes, and at very low levels in a variety of tissues.
  • Pathway

    Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine diphosphate from thiamine: step 1/1.
  • Sequence similarities

    Belongs to the thiamine pyrophosphokinase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • hTPK1 antibody
    • Placental protein 20 antibody
    • PP20 antibody
    • Thiamin pyrophosphokinase 1 antibody
    • Thiamine diphosphokinase antibody
    • Thiamine kinase antibody
    • Thiamine pyrophosphokinase 1 antibody
    • THMD5 antibody
    • TPK1 antibody
    • TPK1_HUMAN antibody
    see all

Images

  • All lanes : Anti-TPK1 antibody (ab230263) at 1/1000 dilution

    Lane 1 : Mouse liver tissue lysate.
    Lane 2 : Mouse kidney tissue lysate.

    Secondary
    All lanes : Goat anti-rabbit IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 27 kDa
    Observed band size: 27 kDa

  • PC-3 (human prostate adenocarcinoma cell line) cells stained for TPK1 (green) using ab230263 at 1/100 dilution in ICC/IF. Secondary used is an Alexa Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).

  • Paraffin-embedded human kidney tissue stained for TPK1 using ab230263 at 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded human heart tissue stained for TPK1 using ab230263 at 1/100 dilution in immunohistochemical analysis.

References

ab230263 has not yet been referenced specifically in any publications.

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