Recombinant
RabMAb

Anti-TPK1 antibody [EPR10918] (ab170863)

Overview

  • Product name
    Anti-TPK1 antibody [EPR10918]
    See all TPK1 primary antibodies
  • Description
    Rabbit monoclonal [EPR10918] to TPK1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide within Human TPK1 aa 200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9H3S4

  • Positive control
    • SH-SY5Y, JAR, Human fetal kidney, Human fetal brain and Human fetal lung lysates. Heart, kidney and tonsi tissue. Immunoprecipitation pellet from fetal brain lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR10918
  • Isotype
    IgG

Associated products

Applications

Our Abpromise guarantee covers the use of ab170863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
WB 1/1000 - 1/5000. Predicted molecular weight: 27 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    Images

    • Western blot analysis on immunoprecipitation pellet from (Lane 1)  fetal brain lysate or (Lane 2) 1X PBS (negative control) using ab170863 at a 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    • Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded Human tonsil tissue labeling TPK1 with ab170863 at a 1/50 dilution.

    • Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded Human kidney tissue labeling TPK1 with ab170863 at a 1/50 dilution.

    • Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded Human heart tissue labeling TPK1 with ab170863 at a 1/50 dilution.

    • All lanes : Anti-TPK1 antibody [EPR10918] (ab170863) at 1/1000 dilution

      Lane 1 : SH-SY5Y lysate
      Lane 2 : JAR lysate
      Lane 3 : Fetal kidney lysate
      Lane 4 : Fetal brain lysate
      Lane 5 : Fetal lung lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 27 kDa

    References

    ab170863 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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