Overview

  • Product name

    Anti-TPM4 antibody - C-terminal
    See all TPM4 primary antibodies
  • Description

    Goat polyclonal to TPM4 - C-terminal
  • Host species

    Goat
  • Specificity

    ab190294 is expected to recognize both reported isoforms of TPM4 (NP_001138632.1; NP_003281.1).
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide within Human TPM4 aa 266-276 (C terminal) (Cysteine residue). The exact sequence is proprietary. NP_001138632.1
    Sequence:

    ENVGLHQTLDQ


    Database link: ab190294

  • Positive control

    • Huma platelets lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 99% Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab190294 purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab190294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).

Target

  • Function

    Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Binds calcium.
  • Tissue specificity

    Detected in cardiac tissue and platelets, the form found in cardiac tissue is a higher molecular weight than the form found in platelets. Expressed at higher levels in the platelets of hypertensive patients with cardiac hypertrophy than in the platelets of hypertensive patients without cardiac hypertrophy (at protein level).
  • Sequence similarities

    Belongs to the tropomyosin family.
  • Domain

    The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • epididymis secretory protein Li 108 antibody
    • HEL-S-108 antibody
    • TM30p1 antibody
    • TMP 4 antibody
    • TPM4 antibody
    • TPM4_HUMAN antibody
    • Tropomyosin 4 antibody
    • Tropomyosin alpha 4 chain antibody
    • Tropomyosin alpha-4 chain antibody
    • Tropomyosin-4 antibody
    • wu:fb06e07 antibody
    • zgc:103436 antibody
    • zgc:63909 antibody
    see all

Images

  • Anti-TPM4 antibody - C-terminal (ab190294) at 0.1 µg/ml (Incubation 1 hour) + NIH3T3 cell Lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 32 kDa

  • Anti-TPM4 antibody - C-terminal (ab190294) at 0.03 µg/ml + Human platelet lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 32 kDa
    Observed band size: 32 kDa


    Exposure time: 1 hour

References

ab190294 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Dog Cell lysate - whole cell (MDCK cells)
Gel Running Conditions
Reduced Denaturing (4-20% gradient gel)
Loading amount
100000 cells
Specification
MDCK cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Dr. Guillaume Charras

Verified customer

Submitted Nov 09 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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