Overview

  • Product name

    Anti-TPM4 antibody - N-terminal
    See all TPM4 primary antibodies
  • Description

    Rabbit polyclonal to TPM4 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide within Human TPM4 aa 26-54 (N terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P67936

  • Positive control

    • IHC-P: Human placenta and skeletal muscle tissues. WB: U-251 MG, A549 and Jurkat cell lysates. Flow Cytometry: U-251 MG cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab230880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 28 kDa.
IHC-P 1/100.
Flow Cyt 1/10 - 1/50.

Target

  • Function

    Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Binds calcium.
  • Tissue specificity

    Detected in cardiac tissue and platelets, the form found in cardiac tissue is a higher molecular weight than the form found in platelets. Expressed at higher levels in the platelets of hypertensive patients with cardiac hypertrophy than in the platelets of hypertensive patients without cardiac hypertrophy (at protein level).
  • Sequence similarities

    Belongs to the tropomyosin family.
  • Domain

    The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • epididymis secretory protein Li 108 antibody
    • HEL-S-108 antibody
    • TM30p1 antibody
    • TMP 4 antibody
    • TPM4 antibody
    • TPM4_HUMAN antibody
    • Tropomyosin 4 antibody
    • Tropomyosin alpha 4 chain antibody
    • Tropomyosin alpha-4 chain antibody
    • Tropomyosin-4 antibody
    • wu:fb06e07 antibody
    • zgc:103436 antibody
    • zgc:63909 antibody
    see all

Images

  • All lanes : Anti-TPM4 antibody - N-terminal (ab230880) at 1/1000 dilution

    Lane 1 : U-251 MG (Human brain glioma cell line) cell lysate
    Lane 2 : A549 (Human lung carcinoma cell line) cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 28 kDa

  • Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for TPM4 using ab230880 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human placenta tissue stained for TPM4 using ab230880 at 1/100 dilution in immunohistochemical analysis.

  • Flow cytometric analysis of U-251 MG (Human brain glioma cell line) cells labeling TPM4 with ab230880 at 1/10 dilution(right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab230880 has not yet been referenced specifically in any publications.

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