Key features and details
- Rabbit polyclonal to TPMT
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TPMT antibody
See all TPMT primary antibodies
DescriptionRabbit polyclonal to TPMT
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla
Recombinant fragment corresponding to Human TPMT aa 4-244.
TRTSLDIEEYSDTEVQKNQVLTLEEWQDKWVNGKTAFHQEQGHQLLKKHL DTFLKGKSGLRVFFPLCGKAVEMKWFADRGHSVVGVEISELGIQEFFTEQ NLSYSEEPITEIPGTKVFKSSSGNISLYCCSIFDLPRTNIGKFDMIWDRG ALVAINPGDRKCYADTMFSLLGKKFQYLLCVLSYDPTKHPGPPFYVPHAE IERLFGKICNIRCLEKVDAFEERHKSWGIDCLFEKLYLLTE
Database link: P51580
- WB: EC109 and HEK-293T whole cell lysates. IHC-P: Human kidney and brain tissues. IP: K562 whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol, PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236648 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 28 kDa.|
|IP||1/200 - 1/2000.|
|IHC-P||1/20 - 1/200.|
FunctionCatalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine.
Involvement in diseaseDefects in TPMT are the cause of thiopurine S-methyltransferase deficiency (TPMT deficiency) [MIM:610460]. TPMT is an enzyme involved in the normal metabolic inactivation of thiopurine drugs. These drugs are generally used as immunosupressants or cytotoxic drugs and are prescribed for a variety of clinical conditions including leukemia, autoimmune disease and organ transplantation. Patients with intermediate or no TPMT activity are at risk of toxicity after receiving standard doses of thiopurine drugs and it is shown that inter-individual differences in response to these drugs are largely determined by genetic variation at the TPMT locus.
Sequence similaritiesBelongs to the methyltransferase superfamily. TPMT family.
- Information by UniProt
- HGNC:12014 antibody
- S adenosyl L methionine thiopurine S methyltransferase antibody
- Thiopurine methyltransferase antibody
All lanes : Anti-TPMT antibody (ab236648) at 1/1000 dilution
Lane 1 : EC109 whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/50000 dilution
Predicted band size: 28 kDa
Paraffin-embedded human kidney tissue stained for TPMT using ab236648 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human brain tissue stained for TPMT using ab236648 at 1/100 dilution in immunohistochemical analysis.
TPMT was immunoprecipitated from 500 µg K562 (human liver chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with ab236648 at 1/200 dilution.
Lane 1: Rabbit control IgG IP in K562 whole cell lysate.
Lane 2: ab236648 IP in K562 whole cell lysate.
Lane 3: K562 whole cell lysate 20 µg (Input).
For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236648 has not yet been referenced specifically in any publications.