Recombinant Anti-TPMT antibody [EPR10820(B)] (ab155284)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10820(B)] to TPMT
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-TPMT antibody [EPR10820(B)]
See all TPMT primary antibodies -
Description
Rabbit monoclonal [EPR10820(B)] to TPMT -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- HAP1, K562, TF1, Jurkat and HepG2 cell lysates; Human brain and kidney tissue; Permeabilized K562 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10820(B) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
|
|
IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
Notes |
---|
Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 28 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
-
Function
Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine. -
Involvement in disease
Defects in TPMT are the cause of thiopurine S-methyltransferase deficiency (TPMT deficiency) [MIM:610460]. TPMT is an enzyme involved in the normal metabolic inactivation of thiopurine drugs. These drugs are generally used as immunosupressants or cytotoxic drugs and are prescribed for a variety of clinical conditions including leukemia, autoimmune disease and organ transplantation. Patients with intermediate or no TPMT activity are at risk of toxicity after receiving standard doses of thiopurine drugs and it is shown that inter-individual differences in response to these drugs are largely determined by genetic variation at the TPMT locus. -
Sequence similarities
Belongs to the methyltransferase superfamily. TPMT family. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 7172 Human
- Entrez Gene: 22017 Mouse
- Entrez Gene: 690050 Rat
- Omim: 187680 Human
- SwissProt: P51580 Human
- SwissProt: O55060 Mouse
- SwissProt: Q9Z0T0 Rat
- Unigene: 444319 Human
see all -
Alternative names
- HGNC:12014 antibody
- S adenosyl L methionine thiopurine S methyltransferase antibody
- Thiopurine methyltransferase antibody
see all
Images
-
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: TPMT knockout HAP1 whole cell lysate (20 µg)
Lane 3: HepG2 whole cell lysate (20 µg)
Lane 4: Jurkat whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab155284 observed at 28 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab155284 was shown to recognize TPMT in wild-type HAP1 cells as signal was lost at the expected MW in TPMT knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TPMT knockout samples were subjected to SDS-PAGE. Ab155284 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 2 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
-
All lanes : Anti-TPMT antibody [EPR10820(B)] (ab155284) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : TF1 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 28 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling TPMT with ab155284 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling TPMT with ab155284 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
Intracellular flow cytometric analysis of permeabilized K562 cells labeling TPMT with ab155284 at 1/100 dilution (red) compared with a rabbit IgG (negative) (green).
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)
Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab155284 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)
Immunohistochemical analysis of paraffin embedded Human gastric adenocarcinoma tissue using ab155284 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)
Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab155284 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab155284 has not yet been referenced specifically in any publications.