Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-TPMT antibody [EPR10820(B)] (ab155284)

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Western blot - Anti-TPMT antibody [EPR10820(B)] (ab155284)
  • Western blot - Anti-TPMT antibody [EPR10820(B)] (ab155284)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)
  • Flow Cytometry - Anti-TPMT antibody [EPR10820(B)] (ab155284)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)
  • Anti-TPMT antibody [EPR10820(B)] (ab155284)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10820(B)] to TPMT
  • Suitable for: WB, IHC-P, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TPMT antibody [EPR10820(B)]
    See all TPMT primary antibodies

  • Description

    Rabbit monoclonal [EPR10820(B)] to TPMT

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    A synthetic peptide, corresponding to residues in Human TPMT (UniProt: P51580).

  • Positive control

    • HAP1, K562, TF1, Jurkat and HepG2 cell lysates; Human brain and kidney tissue; Permeabilized K562 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab155284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine.

  • Involvement in disease

    Defects in TPMT are the cause of thiopurine S-methyltransferase deficiency (TPMT deficiency) [MIM:610460]. TPMT is an enzyme involved in the normal metabolic inactivation of thiopurine drugs. These drugs are generally used as immunosupressants or cytotoxic drugs and are prescribed for a variety of clinical conditions including leukemia, autoimmune disease and organ transplantation. Patients with intermediate or no TPMT activity are at risk of toxicity after receiving standard doses of thiopurine drugs and it is shown that inter-individual differences in response to these drugs are largely determined by genetic variation at the TPMT locus.

  • Sequence similarities

    Belongs to the methyltransferase superfamily. TPMT family.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • HGNC:12014 antibody
    • S adenosyl L methionine thiopurine S methyltransferase antibody
    • Thiopurine methyltransferase antibody
    • Thiopurine S methyltransferase antibody
    • Thiopurine S-methyltransferase antibody
    • TPMT antibody
    • TPMT_HUMAN antibody
    see all

Images

  • Western blot - Anti-TPMT antibody [EPR10820(B)] (ab155284)
    Western blot - Anti-TPMT antibody [EPR10820(B)] (ab155284)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: TPMT knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HepG2 whole cell lysate (20 µg)
    Lane 4: Jurkat whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab155284 observed at 28 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab155284 was shown to recognize TPMT in wild-type HAP1 cells as signal was lost at the expected MW in TPMT knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TPMT knockout samples were subjected to SDS-PAGE. Ab155284 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 2 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-TPMT antibody [EPR10820(B)] (ab155284)
    Western blot - Anti-TPMT antibody [EPR10820(B)] (ab155284)
    All lanes : Anti-TPMT antibody [EPR10820(B)] (ab155284) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : TF1 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 28 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling TPMT with ab155284 at 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling TPMT with ab155284 at 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-TPMT antibody [EPR10820(B)] (ab155284)
    Flow Cytometry - Anti-TPMT antibody [EPR10820(B)] (ab155284)
    Flow cytometric analysis of permeabilized K562 cells labeling TPMT with ab155284 at 1/100 dilution (red) compared with a rabbit IgG (negative) (green).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)

    Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab155284 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)

    Immunohistochemical analysis of paraffin embedded Human gastric adenocarcinoma tissue using ab155284 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)

    Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab155284 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-TPMT antibody [EPR10820(B)] (ab155284)
    Anti-TPMT antibody [EPR10820(B)] (ab155284)

References (0)

Publishing research using ab155284? Please let us know so that we can cite the reference in this datasheet.

ab155284 has not yet been referenced specifically in any publications.

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