Anti-TPMT antibody [EPR10820(B)] (ab155284)
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Overview
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Product nameAnti-TPMT antibody [EPR10820(B)]
See all TPMT primary antibodies -
DescriptionRabbit monoclonal [EPR10820(B)] to TPMT
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC, IP, Flow Cytmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
A synthetic peptide, corresponding to residues in Human TPMT (UniProt: P51580).
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Positive control
- HAP1, K562, TF1, Jurkat and HepG2 cell lysates; Human brain and kidney tissue; Permeabilized K562 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20ºC.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR10820(B)
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IsotypeIgG
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Research areas
Associated products
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab155284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 28 kDa. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC | 1/100 - 1/250. | |
| IP | 1/10 - 1/100. | |
| Flow Cyt | 1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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FunctionCatalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine.
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Involvement in diseaseDefects in TPMT are the cause of thiopurine S-methyltransferase deficiency (TPMT deficiency) [MIM:610460]. TPMT is an enzyme involved in the normal metabolic inactivation of thiopurine drugs. These drugs are generally used as immunosupressants or cytotoxic drugs and are prescribed for a variety of clinical conditions including leukemia, autoimmune disease and organ transplantation. Patients with intermediate or no TPMT activity are at risk of toxicity after receiving standard doses of thiopurine drugs and it is shown that inter-individual differences in response to these drugs are largely determined by genetic variation at the TPMT locus.
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Sequence similaritiesBelongs to the methyltransferase superfamily. TPMT family.
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Cellular localizationCytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 7172 Human
- Entrez Gene: 22017 Mouse
- Entrez Gene: 690050 Rat
- Omim: 187680 Human
- SwissProt: P51580 Human
- SwissProt: O55060 Mouse
- SwissProt: Q9Z0T0 Rat
- Unigene: 444319 Human
see all -
Alternative names
- HGNC:12014 antibody
- S adenosyl L methionine thiopurine S methyltransferase antibody
- Thiopurine methyltransferase antibody
see all
Images
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![Western blot - Anti-TPMT antibody [EPR10820(B)] (ab155284)](https://www.abcam.com/ps/products/155/ab155284/Images/ab155284-297763-ab155284-KO-WB-EWAR.jpg)
Western blot - Anti-TPMT antibody [EPR10820(B)] (ab155284)Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: TPMT knockout HAP1 whole cell lysate (20 µg)
Lane 3: HepG2 whole cell lysate (20 µg)
Lane 4: Jurkat whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab155284 observed at 28 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab155284 was shown to recognize TPMT in wild-type HAP1 cells as signal was lost at the expected MW in TPMT knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TPMT knockout samples were subjected to SDS-PAGE. Ab155284 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 2 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-TPMT antibody [EPR10820(B)] (ab155284)](https://www.abcam.com/ps/products/155/ab155284/Images/TPMT-Primary-antibodies-ab155284-1.jpg)
Western blot - Anti-TPMT antibody [EPR10820(B)] (ab155284)All lanes : Anti-TPMT antibody [EPR10820(B)] (ab155284) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : TF1 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 28 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)](https://www.abcam.com/ps/products/155/ab155284/Images/TPMT-Primary-antibodies-ab155284-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling TPMT with ab155284 at 1/100 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)](https://www.abcam.com/ps/products/155/ab155284/Images/TPMT-Primary-antibodies-ab155284-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling TPMT with ab155284 at 1/100 dilution. -
![Flow Cytometry - Anti-TPMT antibody [EPR10820(B)] (ab155284)](https://www.abcam.com/ps/products/155/ab155284/Images/TPMT-Primary-antibodies-ab155284-4.jpg)
Flow Cytometry - Anti-TPMT antibody [EPR10820(B)] (ab155284)Flow cytometric analysis of permeabilized K562 cells labeling TPMT with ab155284 at 1/100 dilution (red) compared with a rabbit IgG (negative) (green). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)](https://www.abcam.com/ps/products/155/ab155284/Images/TPMT-Primary-antibodies-ab155284-5.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab155284 showing +ve staining. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)](https://www.abcam.com/ps/products/155/ab155284/Images/TPMT-Primary-antibodies-ab155284-6.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)Immunohistochemical analysis of paraffin embedded Human gastric adenocarcinoma tissue using ab155284 showing +ve staining. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)](https://www.abcam.com/ps/products/155/ab155284/Images/TPMT-Primary-antibodies-ab155284-7.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPMT antibody [EPR10820(B)] (ab155284)Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab155284 showing +ve staining.
Protocols
Datasheets and documents
References
ab155284 has not yet been referenced specifically in any publications.
