Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-TPMT antibody [EPR10821(B)] (ab166909)

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Overview

  • Product name

    Anti-TPMT antibody [EPR10821(B)]
    See all TPMT primary antibodies

  • Description

    Rabbit monoclonal [EPR10821(B)] to TPMT

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to residues in Human TPMT (UniProt P51580).

  • Positive control

    • K562, HepG2, Jurkat, TF-1 cell lysate. K562 cells.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab166909 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 28 kDa.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.

    • Target

    • Function

      Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine.

    • Involvement in disease

      Defects in TPMT are the cause of thiopurine S-methyltransferase deficiency (TPMT deficiency) [MIM:610460]. TPMT is an enzyme involved in the normal metabolic inactivation of thiopurine drugs. These drugs are generally used as immunosupressants or cytotoxic drugs and are prescribed for a variety of clinical conditions including leukemia, autoimmune disease and organ transplantation. Patients with intermediate or no TPMT activity are at risk of toxicity after receiving standard doses of thiopurine drugs and it is shown that inter-individual differences in response to these drugs are largely determined by genetic variation at the TPMT locus.

    • Sequence similarities

      Belongs to the methyltransferase superfamily. TPMT family.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • HGNC:12014 antibody
      • S adenosyl L methionine thiopurine S methyltransferase antibody
      • Thiopurine methyltransferase antibody
      • Thiopurine S methyltransferase antibody
      • Thiopurine S-methyltransferase antibody
      • TPMT antibody
      • TPMT_HUMAN antibody
      see all

    Images

    • Western blot - Anti-TPMT antibody [EPR10821(B)] (ab166909)
      Western blot - Anti-TPMT antibody [EPR10821(B)] (ab166909)
      All lanes : Anti-TPMT antibody [EPR10821(B)] (ab166909) at 1/1000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : TPMT knockout HAP1 whole cell lysate
      Lane 3 : HepG2 whole cell lysate
      Lane 4 : K562 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 28 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab166909 observed at 28 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab166909 was shown to recognize TPMT in wild-type HAP1 cells as signal was lost at the expected MW in TPMT knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TPMT knockout samples were subjected to SDS-PAGE. Ab166909 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at  1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-TPMT antibody [EPR10821(B)] (ab166909)
      Western blot - Anti-TPMT antibody [EPR10821(B)] (ab166909)
      All lanes : Anti-TPMT antibody [EPR10821(B)] (ab166909) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : TF-1 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 28 kDa

    • Flow Cytometry - Anti-TPMT antibody [EPR10821(B)] (ab166909)
      Flow Cytometry - Anti-TPMT antibody [EPR10821(B)] (ab166909)
      Flow cytometric analysis of permeabilized K562 cells using ab166909 at a 1/100 dilution (red) or a rabbit IgG (negative) (green).

    References

    ab166909 has not yet been referenced specifically in any publications.

    Publishing research using ab166909? Please let us know so that we can cite the reference in this datasheet.

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