Key features and details
- Rabbit polyclonal to TPN
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TPN antibody
See all TPN primary antibodies
DescriptionRabbit polyclonal to TPN
Weakly cross-reacts with bovine Tapasin.
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Cow
- This antibody gave a positive signal in A431 whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab13518 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).|
FunctionInvolved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading).
Tissue specificityNeutrophils, mostly in fully differentiated cells.
Sequence similaritiesContains 1 Ig-like C1-type (immunoglobulin-like) domain.
DomainThe N-terminus is required for efficient association with MHC class I molecule and for a stable interaction between MHC I and calreticulin. Binding to TAP is mediated by the C-terminus region.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- NGS 17 antibody
- NGS-17 antibody
- NGS17 antibody
Anti-TPN antibody (ab13518) at 1 µg/ml + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 12 minutes
ab13518 has been referenced in 3 publications.
- Wynne JW et al. Characterization of the Antigen Processing Machinery and Endogenous Peptide Presentation of a Bat MHC Class I Molecule. J Immunol 196:4468-76 (2016). PubMed: 27183594
- Del Campo AB et al. Adenovirus expressing ß2-microglobulin recovers HLA class I expression and antitumor immunity by increasing T-cell recognition. Cancer Gene Ther N/A:N/A (2014). PubMed: 24971583
- Chapiro J et al. Destructive cleavage of antigenic peptides either by the immunoproteasome or by the standard proteasome results in differential antigen presentation. J Immunol 176:1053-61 (2006). WB ; Human . PubMed: 16393993