Anti-TPN antibody (ab13518)

Reviews (1)Q&A (1)References (2)

Overview

  • Product name
    Anti-TPN antibody
    See all TPN primary antibodies
  • Description
    Rabbit polyclonal to TPN
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to Human TPN aa 430-448.
    Sequence:

    LGWAAVYLSTCKDSKKKAE

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • This antibody gave a positive signal in A431 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab13518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IP 1/100.

Target

  • Function
    Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading).
  • Tissue specificity
    Neutrophils, mostly in fully differentiated cells.
  • Sequence similarities
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Domain
    The N-terminus is required for efficient association with MHC class I molecule and for a stable interaction between MHC I and calreticulin. Binding to TAP is mediated by the C-terminus region.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • NGS 17 antibody
      • NGS-17 antibody
      • NGS17 antibody
      • TAP associated protein antibody
      • TAP binding protein antibody
      • TAP binding protein (tapasin) antibody
      • TAP BP antibody
      • TAP-associated protein antibody
      • TAP-binding protein antibody
      • TAPA antibody
      • Tapasin antibody
      • TAPBP antibody
      • TPN antibody
      • TPSN antibody
      • TPSN_HUMAN antibody
      see all

    Images

    • Western blot - Anti-TPN antibody (ab13518)
      Western blot - Anti-TPN antibody (ab13518)
      Anti-TPN antibody (ab13518) at 1 µg/ml + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 48 kDa
      Observed band size: 48 kDa


      Exposure time: 12 minutes

    References

    This product has been referenced in:
    • Del Campo AB  et al. Adenovirus expressing ß2-microglobulin recovers HLA class I expression and antitumor immunity by increasing T-cell recognition. Cancer Gene Ther N/A:N/A (2014). Read more (PubMed: 24971583) »
    • Chapiro J  et al. Destructive cleavage of antigenic peptides either by the immunoproteasome or by the standard proteasome results in differential antigen presentation. J Immunol 176:1053-61 (2006). WB ; Human . Read more (PubMed: 16393993) »
    See all 2 Publications for this product

    Publishing research using ab13518? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-TPN antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human Fibroblast cell line)
    Gel Running Conditions
    Reduced Denaturing (12% gel)
    Loading amount
    100 µg
    Specification
    Human Fibroblast cell line
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 18 2012

    Question

    Our end user sent claim report again. He want to receive credit. Please check this claim report and figure and let me know how to I take care. Claim Report Product name Anti-tapasin ab13518 Batch no: 65676 * Research Purpose Immunoprecipitation 1. Antibody Storage Conditions (temperature/reconstitution etc) -20 C 2. Description of the problem (high background, wrong band size, more bands, no band etc.) No band 3. Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.) HeLa cell hole lysate 4. Sample preparation (Buffer/Protease inhibitors/Heating sample etc.) 1 % triton X-100 in PBS 5. Amount of protein loaded 500 ug 6. Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.) 10 % SDS PAGE gel in reducing condition 7. Transfer and blocking conditions (Buffer/Time period, Blocking agent etc.) General transfer buffer / 90 volt / 1 hour / PBS-T containing 5 % skim milk 8. Primary antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, wash step) immunoblot à [a competitor] / goat / 1:800 / 12 hours / three times for 10 min (PBS-T) 9. Secondary antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, wash step) [a competitor] / anti-goat / 1:5000 / 1 hour / three times for 15 min (PBS-T) 10. Detection method (ECL, ECL Plus etc.) Pierce, super signal 11. Positive and negative controls used (Please specify) Goat anti-tapasin 12. How many times have you tried the Western? Three times 13. Have you run a “No Primary” control? (Yes or No) No 14. Do you obtain the same results every time? (Yes or No) Yes 15. What steps have you altered? Dilution / incubation time 16. Additional Notes Control : [a competitor] antibody

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    Answer

    I'm sorry to hear your customer is having problems with ab13518 and thank him for taking the time to give us protocol details. We have not received any complaints regarding this antibody and are confused by the complaint form: the customer mentions immunoprecipitation as the application used for this antibody. However, from the protocol it appears that the immunoprecipitation step was done with the competitor antibody and no details for the Ip were provided. If this is correct we suggest testing our antibody on lysates of HeLa cells by western blotting. A 1:1,000 dilution of ab13518 is sufficent for detection of Tapasin in 20µg of HeLa cell lysate and will determine whether the antibody is faulty. Thank you for sending us an image, however it is unclear to us what it shows as there is no legend. Is it comparing two immunoprecipitations with different antibodies and reprobe with the same antibody? We would like to emphasise to use an adequate lysis buffer (e.g RIPA buffer) with a cocktail of protease inhibitors to prevent the degradation of the protein rather than PBST. Samples should be mixed with loading buffer (containing SDS and betamercaptoethanol) and boiled for 5min at 95C to unfold and denature the protein for adequate recognition. As this information was not present on the form we wish to confirm this recommendation. I apologise for asking clarification of the problem. We have updated our questionnaires for our distributors and can send you those so that we can obtain the maximum information from the customer initially and help the customer as quickly as possible (for example we have an immunoprecipitation specific questionnaire). Please let us know which e-mail address you would like the forms to be sent to you and I will send those immediately. Thank you for your patience, we look forward to hearing from you,

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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