Overview

  • Product name

    Anti-TPOR/MPL antibody [EPR4194]
    See all TPOR/MPL primary antibodies
  • Description

    Rabbit monoclonal [EPR4194] to TPOR/MPL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human TPOR/MPL aa 600 to the C-terminus.
    Database link: P40238

  • Positive control

    • HepG2, A431, and Human marrow lysates.
  • General notes

     

     

     This product was previously labelled as TPOR

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4194
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 71 kDa.

Target

  • Function

    Receptor for thrombopoietin. May represent a regulatory molecule specific for TPO-R-dependent immune responses.
  • Tissue specificity

    Expressed at a low level in a large number of cells of hematopoietic origin. Isoform 1 and isoform 2 are always found to be coexpressed.
  • Involvement in disease

    Defects in MPL are a cause of congenital amegakaryocytic thrombocytopenia (CAMT) [MIM:604498]. CAMT is a disease characterized by isolated thrombocytopenia and megakaryocytopenia with no physical anomalies.
  • Sequence similarities

    Belongs to the type I cytokine receptor family. Type 1 subfamily.
    Contains 2 fibronectin type-III domains.
  • Domain

    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • C MPL antibody
    • CD110 antibody
    • mpl antibody
    • MPLV antibody
    • Myeloproliferative leukemia protein antibody
    • Myeloproliferative leukemia virus oncogene antibody
    • Proto-oncogene c-Mpl antibody
    • THCYT2 antibody
    • Thrombopoietin receptor antibody
    • TPO R antibody
    • TPO-R antibody
    • TPOR antibody
    • TPOR_HUMAN antibody
    see all

Images

  • Anti-TPOR/MPL antibody [EPR4194] (ab109003) at 1/1000 dilution (purified) + mouse spleen lysate at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 71 kDa
    Observed band size: 82 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • All lanes : Anti-TPOR/MPL antibody [EPR4194] (ab109003) at 1/5000 dilution (purified)

    Lane 1 : K562 cell lysate
    Lane 2 : rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

    Predicted band size: 71 kDa
    Observed band size: 82 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Anti-TPOR/MPL antibody [EPR4194] (ab109003) at 1/2500 dilution (purified) + A431 cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

    Predicted band size: 71 kDa
    Observed band size: 82 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • All lanes : Anti-TPOR/MPL antibody [EPR4194] (ab109003) at 1/1000 dilution (unpurified)

    Lane 1 : HepG2 cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : Human marrow lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 71 kDa

References

This product has been referenced in:

  • Yan Z  et al. CD110 promotes pancreatic cancer progression and its expression is correlated with poor prognosis. J Cancer Res Clin Oncol 145:1147-1164 (2019). Read more (PubMed: 30770989) »
See 1 Publication for this product

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