Overview

  • Product name
  • Description
    Mouse monoclonal to TPP1
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 195-305 of Human TPP1

  • Positive control
    • IHC-P: Human salivary gland tissue. WB: A431 cell lysate. FC: JEG3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab54685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 61 kDa.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Lysosomal serine protease with tripeptidyl-peptidase I activity. May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases. Requires substrates with an unsubstituted N-terminus.
  • Tissue specificity
    Detected in all tissues examined with highest levels in heart and placenta and relatively similar levels in other tissues.
  • Involvement in disease
    Defects in TPP1 are the cause of neuronal ceroid lipofuscinosis type 2 (CLN2) [MIM:204500]. A form of neuronal ceroid lipofuscinosis. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy. The lipopigment pattern seen most often in CLN2 consists of curvilinear profiles.
  • Sequence similarities
    Belongs to the peptidase S53 family.
  • Post-translational
    modifications
    Activated by autocatalytic proteolytical processing upon acidification. N-glycosylation is required for processing and activity.
  • Cellular localization
    Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell growth inhibiting gene 1 protein antibody
    • Cell growth-inhibiting gene 1 protein antibody
    • Ceroid lipofuscinosis neuronal 2 antibody
    • Ceroid lipofuscinosis neuronal 2 late infantile (Jansky Bielschowsky disease) antibody
    • Ceroid lipofuscinosis neuronal 2 late infantile antibody
    • CLN 2 antibody
    • CLN2 antibody
    • GIG 1 antibody
    • GIG1 antibody
    • Growth inhibiting protein 1 antibody
    • LPIC antibody
    • Lysosomal pepstatin insensitive protease antibody
    • Lysosomal pepstatin-insensitive protease antibody
    • MGC21297 antibody
    • TPP 1 antibody
    • TPP I antibody
    • TPP-1 antibody
    • TPP-I antibody
    • Tpp1 antibody
    • TPP1_HUMAN antibody
    • TPPI antibody
    • Tripeptidyl aminopeptidase antibody
    • Tripeptidyl peptidase I antibody
    • Tripeptidyl-peptidase 1 antibody
    • Tripeptidyl-peptidase I antibody
    see all

Images

  • TPP1 antibody (ab54685) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human salivary gland.
  • TPP1 antibody (ab54685) at 1ug/lane + A-431 cell lysate at 25ug/lane.
  • Overlay histogram showing JEG3 cells stained with ab54685 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54685, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References

This product has been referenced in:
  • Lee JH  et al. Loss of RNA-binding protein HuR facilitates cellular senescence through posttranscriptional regulation of TIN2 mRNA. Nucleic Acids Res 46:4271-4285 (2018). Read more (PubMed: 29584879) »
  • Sima N  et al. Neural stem cells for disease modeling and evaluation of therapeutics for infantile (CLN1/PPT1) and late infantile (CLN2/TPP1) neuronal ceroid lipofuscinoses. Orphanet J Rare Dis 13:54 (2018). WB . Read more (PubMed: 29631617) »
See all 9 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Heart, Left Ventricle)
Loading amount
40 µg
Specification
Heart, Left Ventricle
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dawn Bowles

Verified customer

Submitted Dec 28 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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