Anti-TPP1 antibody (ab96498)
Key features and details
- Rabbit polyclonal to TPP1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TPP1 antibody
See all TPP1 primary antibodies -
Description
Rabbit polyclonal to TPP1 -
Host species
Rabbit -
Specificity
This product detects Tripeptidyl-peptidase 1 (TPP1). It is unable to detect Adrenocortical dysplasia protein homolog which is also known as TPP1.
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog
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Immunogen
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 224 and 562 of TPP1 (NP_000382)
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Positive control
- A431 whole cell lysate and H1299, HeLa, HepG2 lysates
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab96498 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 61 kDa. |
Target
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Function
Lysosomal serine protease with tripeptidyl-peptidase I activity. May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases. Requires substrates with an unsubstituted N-terminus. -
Tissue specificity
Detected in all tissues examined with highest levels in heart and placenta and relatively similar levels in other tissues. -
Involvement in disease
Defects in TPP1 are the cause of neuronal ceroid lipofuscinosis type 2 (CLN2) [MIM:204500]. A form of neuronal ceroid lipofuscinosis. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy. The lipopigment pattern seen most often in CLN2 consists of curvilinear profiles. -
Sequence similarities
Belongs to the peptidase S53 family. -
Post-translational
modificationsActivated by autocatalytic proteolytical processing upon acidification. N-glycosylation is required for processing and activity. -
Cellular localization
Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 515575 Cow
- Entrez Gene: 485337 Dog
- Entrez Gene: 1200 Human
- Entrez Gene: 12751 Mouse
- Entrez Gene: 83534 Rat
- Omim: 607998 Human
- SwissProt: Q0V8B6 Cow
- SwissProt: Q9XSB8 Dog
see all -
Alternative names
- Cell growth inhibiting gene 1 protein antibody
- Cell growth-inhibiting gene 1 protein antibody
- Ceroid lipofuscinosis neuronal 2 antibody
see all
Images
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Western blot - Anti-TPP1 antibody (ab96498)Anti-TPP1 antibody (ab96498) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 61 kDa
7.5% SDS Page
References (2)
ab96498 has been referenced in 2 publications.
- Shlevkov E et al. A High-Content Screen Identifies TPP1 and Aurora B as Regulators of Axonal Mitochondrial Transport. Cell Rep 28:3224-3237.e5 (2019). PubMed: 31533043
- Chandra S et al. Aspirin Induces Lysosomal Biogenesis and Attenuates Amyloid Plaque Pathology in a Mouse Model of Alzheimer's Disease via PPARa. J Neurosci 38:6682-6699 (2018). PubMed: 29967008
