Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Mouse Cell lysate - other (Brain)
Specification
Brain
Blocking step
Li-Cor Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Li-Cor Blocking Buffer
Dilution
1/600
Secondary antibody
Name
Non-Abcam antibody was used: Li-Cor IRdye
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye 680LT
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye 680LT
Dilution
1/17000
Detection
Detection method
IR
Bands
Specific: ~55 kDa
Exposure
6 minute(s) and 0 second(s)
Abcam user community
Verified customer
Submitted Jan 22 2015